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. 2017 Sep;41(3):707-712.
doi: 10.1007/s12639-016-0871-5. Epub 2016 Dec 24.

Alterations in oxidative stress parameters and its associated correlation with clinical disease on experimental Cryptosporidium parvum infection in Swiss albino mice

Meenakshi Bhagat  1 , Shilpa Sood  1 , Anish Yadav  2 , Pawan Verma  3 , Nasir Manzoor  3 , D Chakraborty  4 , Rajesh Katoch  2 , Navrose Sangha  1
Affiliations

Alterations in oxidative stress parameters and its associated correlation with clinical disease on experimental Cryptosporidium parvum infection in Swiss albino mice

Meenakshi Bhagat et al. J Parasit Dis. 2017 Sep.

Abstract

The present study was conducted to evaluate the changes in oxidative stress parameters on experimental infection with Cryptosporidium parvum in Swiss albino mice. The mice were divided into four groups viz., group I-IV, each group comprising of 15 mice. Group I mice served as healthy control. In Group II mice, C. parvum oocysts @ 104/os were administered, mice of group III were given dexamethasone @ 30 µg/ml in drinking water whereas group IV mice were given dexamethasone @ 30 µg/ml along with C. parvum oocysts @ 104/os. Significant changes were seen in oxidative stress parameters which included significant increase in LPO and decrease in levels of SOD, CAT and GSH in liver and intestine in group IV mice at 10th DPI when compared to others indicating an important role played by free radical induced oxidative stress in the development of C. parvum infection in mice which was clinically characterized by loss of body condition, profuse bloody diarrhoea and peak oocyst shedding intensity occurring at 10th DPI.

Keywords: CAT; Cryptosporidium parvum; GSH; LPO; Oxidative stress; SOD.

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Conflict of interest statement

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The institutional ethics committee for animal experiments from the Sher-e- Kashmir University of Agricultural Sciences and Technology of Jammu granted an approval for the conduct of the present work (Order No. 862/ac/04/CPCSEA).

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