Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:975 Pt 2:1179-1190.
doi: 10.1007/978-94-024-1079-2_94.

Taurine Attenuates Doxorubicin-Induced Toxicity on B16F10 Cells

Yon-Suk Kim #  1   2 , Eun-Kyung Kim #  3 , Jin-Woo Hwang  1 , Won-Suk Kim  4 , Woen-Bin Shin  1 , Sithranga Boopathy Natarajan  1 , Sang-Ho Moon  2 , Byong-Tae Jeon  2 , Pyo-Jam Park  5   6
Affiliations

Taurine Attenuates Doxorubicin-Induced Toxicity on B16F10 Cells

Yon-Suk Kim et al. Adv Exp Med Biol. 2017.

Abstract

This study aimed to investigate the effect of doxorubicin co-treatment with taurine on B16F10 melanoma cells. Frequently, Doxorubicin is used in the treatments of many different kinds of cancers, some of which are soft tissue sarcomas, hematological malignancies and carcinomas. However, the clinical application of doxorubicin is compromised by its severe adverse effects, including cardiotoxicity. In the present study, the efficacy of doxorubicin co-treatment with taurine was investigated. B16F10 cell viability was evaluated using MTT assays, trypan blue dye exclusion assays, and fluorescent staining technique. Apoptotic cells were detected by flow cytometry and the proteins associated with apoptosis and cellular differentiations were assessed by immunoblotting. Doxorubicin inhibited cell growth and induced cell death in B16F10 cells. Interestingly, doxorubicin co-treatment with taurine inhibited apoptosis in B16F10 cells. These results indicate that doxorubicin co-treatment with taurine attenuates doxorubicin-induced cytotoxicity and reduces ROS production in B16F10 cells.

Keywords: B16F10 cells; Doxorubicin; Reactive oxygen species.

PubMed Disclaimer

References

    1. Abdih H, Kelly CJ, Bouchier-Hayes D, Barry M, Kearns S (2000) Taurine prevents interleukin-2-induced acute lung injury in rats. Eur Surg Res 32:347–352 - DOI - PubMed
    1. Awasthi S, Sharma R, Singhal SS, Zimniak P, Awasthi YC (2002) RLIP76, a novel transporter catalyzing ATP-dependent efflux of xenobiotics. Drug Metab Dispos 30:1300–1310 - DOI - PubMed
    1. Buttke TM, Sandstrom PA (1994) Oxidative stress as a mediator of apoptosis. Immunol Today 15:7–10 - DOI - PubMed
    1. Condron C, Casey RG, Kehoe S, Toomey D, Creagh T, Bouchier-Hayes DJ (2010) Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder. Urol Res 38:215–222 - DOI - PubMed
    1. Chen WQ, Jin H, Nguyen M, Carr J, Lee YJ, Hsu CC, Faiman MD, Schloss JV, Wu JY (2001) Role of taurine in regulation of intracellular calcium level and neuroprotective function in cultured neurons. J Neurosci Res 66:612–619 - DOI - PubMed

Publication types

LinkOut - more resources