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. 2017 Jul;20(7):835-840.
doi: 10.22038/IJBMS.2017.9022.

Staging of colorectal cancer using serum metabolomics with 1HNMR Spectroscopy

Farideh Vahabi  1 , Sedigheh Sadeghi  2 , Mohammad Arjmand  1 , Fatemeh Mirkhani  1 , Eshagh Hosseini  2 , Mahsheed Mehrabanfar  3 , Reza Hajhosseini  3 , Ayda Iravani  1 , Parastou Bayat  1 , Zahra Zamani  1
Affiliations

Staging of colorectal cancer using serum metabolomics with 1HNMR Spectroscopy

Farideh Vahabi et al. Iran J Basic Med Sci. 2017 Jul.

Abstract

Objectives: Determination of stages of colon cancer is done by biopsy usually after surgery. Metabolomics is the study of all the metabolites using LC-MS and 1HNMR spectroscopy with chemometric techniques. The stages of colon cancer were detected from patients' sera using 1HNMR.

Materials and methods: Five ml blood was collected from 16 confirmed patients referred for colonoscopy. One group of eight patients were diagnosed with stage 0 to I colon cancer and the second group of 8 patients with II-IV stage colon cancer. Sera were sent for 1HNMR. The differentiating metabolites were identified using HMDB and the metabolic cycles from Metaboanalyst.

Results: Six metabolites of which pyridoxine levels lowered, and glycine, cholesterol, taurocholic acid, cholesteryl ester and deoxyinosine increased.

Conclusion: The different stages of cancer were identified by the main metabolic cycles such as primary bile acid biosynthesis, purine and vitamin B metabolic pathways and the glutathione cycle.

Keywords: 1HNuclear Magnetic -Resonance Spectroscopy; Colon cancer; Metabolomics; Serum; Staging.

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Figures

Figure 1
Figure 1
Spectra of the two groups with differentiated metabolites; horizontal axis depicting chemical shifts of the metabolites in ppm with TMSP as reference and y axis shows signal intensitiesproportional to molar concentrations
Figure 2
Figure 2
Score plot of PLS after OSC shows very good separation of samples; odd numbers indicate 0, I colon cancer stages and even numbers II, III and IV colon cancer stages samples
Figure 3
Figure 3
Loading plot with levels of differentiating metabolites in the two groups
Figure 4
Figure 4
Plot of Fold Enrichment Analysis showing the main metabolic pathways affected in the two groups of colon cancer stages
See this image and copyright information in PMC

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