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. 2017 Jul-Aug;7(4):197-201.
doi: 10.4103/jispcd.JISPCD_90_17. Epub 2017 Jul 31.

Isolation and Identification of Nonoral Pathogenic Bacteria in the Oral Cavity of Patients with Removable Dentures

Reza Derafshi  1 , Abdollah Bazargani  2 , Jannan Ghapanchi  3 , Yazdan Izadi  3 , Hooman Khorshidi  4
Affiliations

Isolation and Identification of Nonoral Pathogenic Bacteria in the Oral Cavity of Patients with Removable Dentures

Reza Derafshi et al. J Int Soc Prev Community Dent. 2017 Jul-Aug.

Abstract

Aims and objectives: Dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. The aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in Shiraz, Southern Iran.

Materials and methods: The bacterial flora of saliva samples from 50 men and 50 women with removable dentures and 100 age- and sex-matched controls with normal dentate were compared using culture, Gram staining, and API20E Kit methods. All data were analyzed using SPSS software.

Results: Except for Enterobacter cloacae isolate (P = 0.03), there was no significant difference between both groups for the presence of Escherichia coli, Klebsiella pneumoniae, nonfermenting Gram-negative bacilli, Raoultella ornithinolytica, Raoultella planticola, Kluyvera spp., and Enterobacter aerogenes. No significant correlation was noticed between age and presence of bacteria in the oral cavity. The Gram-negative rod bacteria were more in males, but the difference was not significant. When a total of Gram-negative rods were considered, there was a significant difference between case and control groups (P = 0.004).

Conclusions: Based on our findings that nonoral pathogenic bacteria are detected from the saliva of the denture wearers, general and oral health measures in patients with removable dentures should be adopted to decrease the risk of cross infection.

Keywords: Dental prosthesis; Gram-negative bacilli; nonoral pathogenic bacteria; oral cavity; saliva.

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Conflict of interest statement

There are no conflicts of interest.

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