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. 2016 Jul 3;13(4):184-190.
doi: 10.21010/ajtcam.v13i4.24. eCollection 2016.

INHIBITORY EFFECT OF LYCOPENE AGAINST THE GROWTH OF HUMAN GASTRIC CANCER CELLS

ShenKang Zhou  1 , RuiLi Zhang  1 , TieNan Bi  1 , Yong Lu  1 , LiangXian Jiang  1
Affiliations

INHIBITORY EFFECT OF LYCOPENE AGAINST THE GROWTH OF HUMAN GASTRIC CANCER CELLS

ShenKang Zhou et al. Afr J Tradit Complement Altern Med. .

Abstract

Background: The aim of this study was to investigate the anti-proliferative effect of Lycopene on HGC-27 cells.

Materials and methods: HGC-27 cells were treated with varying concentration lycopene for 24, 48, 72 h. The cell growth inhibition was analyzed by MTT. Western blotting was used to indicate changes in the levels of LC3-I, LC3-II, ERK (extracellular signal-regulated protein kinase) and phosphorylation-ERK (p-ERK).

Results: Lycopene displayed antiproliferative activity in HGC-27 cell lines. Western blotting showed that Lycopene significantly enhanced LC3-I, p-ERK proteins expression. In gastric cancer nude mice model, lycopene treatment significantly decreased tumour weight. These findings indicated that lycopene treatment induces the anti-proliferation of HGC-27 cells.

Conclusion: Lycopene treatment inhibited HGC-27 cells growth by activating ERK.

Keywords: Apoptosis; HGC-27; gastric cancer; p-ERK.

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Figures

Figure 1
Figure 1
Effect of lycopene treatment on cell morphology
Figure 2
Figure 2
The effect of lycopene on the proliferation of HGC-27 cells at 24 h, 48 h and 72 h of culture. Control, HGC-27 cells without lycopene. Data are represented as means±SEM of three independent experiments. Statistical significance versus control: *P<0.05, **P<0.001.
Figure 3
Figure 3
Western bloting for LC3-II in HGC-27 cells at 72h after lycopene or HCQ treatment 1. control, 2. Lycopene (20 μmol/l), 3. Lycopene (45 μmol/l), 4. HCQ (40 μmol/l)
Figure 4
Figure 4
Western bloting for ERK and p-ERK in HGC-27 cells at 72h after lycopene treatment.
Figure 5
Figure 5
Effect of lycopene treatment on tumour volume in the nude mice gastric cancer model. *P<0.05, **P<0.01, compared with vehicle control
Figure 6
Figure 6
Effect of lycopene treatment on tumour weight in the nude mice gastric cancer model. **P<0.01, compared with vehicle control
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