Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Oct;35(10):936-939.
doi: 10.1038/nbt.3973. Epub 2017 Aug 30.

Multiplexed quantification of proteins and transcripts in single cells

Vanessa M Peterson  1 , Kelvin Xi Zhang  2 , Namit Kumar  1 , Jerelyn Wong  3 , Lixia Li  1 , Douglas C Wilson  3 , Renee Moore  4 , Terrill K McClanahan  3 , Svetlana Sadekova  3 , Joel A Klappenbach  1
Affiliations

Multiplexed quantification of proteins and transcripts in single cells

Vanessa M Peterson et al. Nat Biotechnol. 2017 Oct.

Abstract

We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.

PubMed Disclaimer

References

    1. Bioinformatics. 2012 Jul 15;28(14):1933-4 - PubMed
    1. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50 - PubMed
    1. Science. 2017 Apr 21;356(6335): - PubMed
    1. BMC Bioinformatics. 2011 Aug 04;12:323 - PubMed
    1. J Immunother Cancer. 2015 Aug 18;3:37 - PubMed

MeSH terms