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. 2017 Oct 15:242:49-57.
doi: 10.1016/j.virusres.2017.08.012. Epub 2017 Sep 21.

Genomes of viral isolates derived from different mosquitos species

Mohammadreza Sadeghi  1 , Vsevolod Popov  2 , Hilda Guzman  2 , Tung Gia Phan  3 , Nikos Vasilakis  4 , Robert Tesh  4 , Eric Delwart  5
Affiliations

Genomes of viral isolates derived from different mosquitos species

Mohammadreza Sadeghi et al. Virus Res. .

Abstract

Eleven viral isolates derived mostly in albopictus C6/36 cells from mosquito pools collected in Southeast Asia and the Americas between 1966 and 2014 contained particles with electron microscopy morphology typical of reoviruses. Metagenomics analysis yielded the near complete genomes of three novel reoviruses, Big Cypress orbivirus, Ninarumi virus, and High Island virus and a new tetravirus, Sarawak virus. Strains of previously characterized Sathuvarachi, Yunnan, Banna and Parry's Lagoon viruses (Reoviridae), Bontang virus (Mesoniviridae), and Culex theileri flavivirus (Flaviviridae) were also characterized. The availability of these mosquito virus genomes will facilitate their detection by metagenomics or PCR to better determine their geographic range, extent of host tropism, and possible association with arthropod or vertebrate disease.

Keywords: Deep sequencing; Metagenomics; Mosquito; Orbivirus; Reoviridae; Viral discovery.

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Conflict of interest statement

Competing interests

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
A. Phylogeny of the Orbivirus and Idnoreovirus genera based on the RdRp gene (VP1). B. Phylogeny of Orbiviruses based on innermost protein capsid (VP3). C. Tetravirus genome open reading frames and phylogeny of ORF1 protein.
Figure 1
Figure 1
A. Phylogeny of the Orbivirus and Idnoreovirus genera based on the RdRp gene (VP1). B. Phylogeny of Orbiviruses based on innermost protein capsid (VP3). C. Tetravirus genome open reading frames and phylogeny of ORF1 protein.
Figure 2
Figure 2
Electron microscopy of ten viral isolates included in this study. A. Big Cypress virus; B. Ninarumi virus; C. Parry’s Lagoon virus; D. Yunnan virus (JKT-10087); E. Banna virus; F. High Island orbivirus; G. Bontang virus; H. Yunnan virus (JKT-8650); I. Sathuvachari virus; J. Sarawak tetravirus. Bars = 100 nm
Figure 2
Figure 2
Electron microscopy of ten viral isolates included in this study. A. Big Cypress virus; B. Ninarumi virus; C. Parry’s Lagoon virus; D. Yunnan virus (JKT-10087); E. Banna virus; F. High Island orbivirus; G. Bontang virus; H. Yunnan virus (JKT-8650); I. Sathuvachari virus; J. Sarawak tetravirus. Bars = 100 nm
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References

    1. Aguiar ER, Olmo RP, Paro S, Ferreira FV, de Faria IJ, Todjro YM, Lobo FP, Kroon EG, Meignin C, Gatherer D, Imler JL, Marques JT. Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host. Nucleic acids research. 2015;43(13):6191–6206. - PMC - PubMed
    1. Attoui H, Mertens PPC, Becnel J, Belaganahalli S, Bergoin M, Brussaard CP, Chappell JD, Ciarlet M, del Vas M, TSD . Virus Taxonomy, Ninth Report of the International Committee on Taxonomy of Viruses. x. Academic Press; London; Waltham, MA: 2012. p. 1327.
    1. Attoui H, Billoir F, Biagini P, de Micco P, de Lamballerie X. Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae. The Journal of general virology. 2000;81(Pt 6):1507–1515. - PubMed
    1. Attoui H, Mendez-Lopez MR, Rao S, Hurtado-Alendes A, Lizaraso-Caparo F, Jaafar FM, Samuel AR, Belhouchet M, Pritchard LI, Melville L, Weir RP, Hyatt AD, Davis SS, Lunt R, Calisher CH, Tesh RB, Fujita R, Mertens PP. Peruvian horse sickness virus and Yunnan orbivirus, isolated from vertebrates and mosquitoes in Peru and Australia. Virology. 2009;394(2):298–310. - PubMed
    1. Attoui H, Mohd Jaafar F. Zoonotic and emerging orbivirus infections. Rev Sci Tech. 2015;34(2):353–361. - PubMed