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. 2017 Sep;24(6):1268-1273.
doi: 10.1016/j.sjbs.2016.01.001. Epub 2016 Jan 7.

Ecological variations and role of heat shock protein in Artemisia judaica L. in response to temperature regimes of Tabuk, Saudi Arabia

Zahid Khorshid Abbas  1 , Shalini Saggu  1 , Hasibur Rehman  1 , Aziz Al Thbiani  1 , Abid A Ansari  1
Affiliations

Ecological variations and role of heat shock protein in Artemisia judaica L. in response to temperature regimes of Tabuk, Saudi Arabia

Zahid Khorshid Abbas et al. Saudi J Biol Sci. 2017 Sep.

Abstract

Artemisia judaica L. (Compositae) are shrubby herbs growing wildly in Tabuk region and distributed in the desert regions. This region is characterized by extremely variable environmental conditions where the temperature varies from extreme low to extreme high. These temperature regimes have a profound effect on morphology, growth physiology and biochemistry of the plants. The plant samples were collected from Tabuk-Jordan road (760 m above sea level) in the month of January, April, July and October 2013 to evaluate the effect of temperature dynamics on A. judaica L. in four different seasons. Physiological, biochemical alterations and heat shock proteins (HSPs) were studied during these seasons in order to evaluate the environmental adaptation and stress tolerance in response to temperature variations. Plant growth parameters showed a significant increase in height, fresh and dry matter accumulation, total chlorophyll, nitrogen, phosphorus, potassium, artemisinin and leaf relative water contents investigated in the month of April and October. Growth of plant was suppressed and an active role of carbonic anhydrase (CA), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) was observed to cope with the extreme low temperature in January and extreme high temperature in July 2013. However, the plants collected in October and April did not show a statistical difference. Inductions in the expression of HSP90 were recorded in all the plants collected during April and October 2013 with no statistically significant difference. Therefore, based on the results it is recommended that during April and October the environmental conditions are best suitable for growth, development and medicinal use of Artemisia.

Keywords: Antioxidants; Climate change; Growth; Heat shock proteins; Medicinal plants.

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Figures

Figure 1
Figure 1
CA activity of Artemisia judaica in response to temperature regimes of Tabuk, Saudi Arabia.
Figure 2
Figure 2
Variation in antioxidant enzyme activities of Artemisia judaica to temperature regimes of Tabuk, Saudi Arabia. (A) enzymatic activity of SOD, (B) activity of CAT and C. POD activity.
Figure 3
Figure 3
Expression of HSP 90 of Artemisia judaica in response to temperature regimes of Tabuk, Saudi Arabia.
See this image and copyright information in PMC

References

    1. Asada K. The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Rev. Plant Physiol. Plant Mol. Biol. 1999;50:601–639. - PubMed
    1. Awasthi R., Bhandari K., Nayyar H. Temperature stress and redox homeostasis in agricultural crops. Front. Environ. Sci. 2015;3(3):1–24.
    1. Beauchamp C., Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Annal. Biochem. 1971;44:276–287. - PubMed
    1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annal. Biochem. 1976;72:248–254. - PubMed
    1. Cakmak I., Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant Physiol. 1992;98:1222–1227. - PMC - PubMed

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