GUIDES: sgRNA design for loss-of-function screens

Joshua A Meier^{1

2

3

4}, Feng Zhang^{1

2}, Neville E Sanjana^{3

4}

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
² McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
³ New York Genome Center, New York, New York, USA.
⁴ Department of Biology, New York University, New York, New York, USA.

PMID: 28858339
PMCID: PMC5870754
DOI: 10.1038/nmeth.4423

GUIDES: sgRNA design for loss-of-function screens

Joshua A Meier et al. Nat Methods. 2017.

. 2017 Aug 31;14(9):831-832.

doi: 10.1038/nmeth.4423.

Authors

Joshua A Meier^{1

2

3

4}, Feng Zhang^{1

2}, Neville E Sanjana^{3

4}

Affiliations

¹ Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
² McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
³ New York Genome Center, New York, New York, USA.
⁴ Department of Biology, New York University, New York, New York, USA.

PMID: 28858339
PMCID: PMC5870754
DOI: 10.1038/nmeth.4423

No abstract available

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Conflict of interest statement

Competing financial interests

A patent has been filed relating to the described work. F.Z. is a founder and scientific advisor for Editas Medicine, and a scientific advisor for Horizon Discovery.

Figures

**Figure 1. GUIDES design environment**
Screenshot of interactive designer for adding and deleting genes and sgRNAs.

See this image and copyright information in PMC

References

1. Shalem O, Sanjana NE, Zhang F. High-throughput functional genomics using CRISPR-Cas9. Nat Rev Genet. 2015;16:299–311. - PMC - PubMed
1. GTEx Consortium. Human genomics. The Genotype-Tissue Expression (GTEx) pilot analysis: multitissue gene regulation in humans. Science. 2015;348:648–660. - PMC - PubMed
1. Murphy D, Singh R, Kolandaivelu S, Ramamurthy V, Stoilov P. Alternative Splicing Shapes the Phenotype of a Mutation in BBS8 To Cause Nonsyndromic Retinitis Pigmentosa. Mol Cell Biol. 2015;35:1860–1870. - PMC - PubMed
1. Doench JG, et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat Biotechnol. 2016;34:184–191. - PMC - PubMed
1. Shi J, et al. Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nat Biotechnol. 2015;33:661–667. - PMC - PubMed

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GUIDES: sgRNA design for loss-of-function screens

Affiliations

GUIDES: sgRNA design for loss-of-function screens

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Conflict of interest statement

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