Data describing IFNγ-mediated viral clearance in an adult mouse model of respiratory syncytial virus (RSV)

Abstract

The data presented here are related to the research article entitled "Age predicts cytokine kinetics and innate immune cell activation following intranasal delivery of IFNγ and GM-CSF in a mouse model of RSV infection" (Eichinger et al., 2017) [1]. The cited manuscript demonstrated that the macrophage-stimulating cytokine, interferon gamma (IFNγ), but not granulocyte macrophage-colony stimulating factor (GM-CSF), effectively enhanced viral clearance in infant mice infected with respiratory syncytial virus (RSV) following intranasal delivery. This article describes the immune response and viral clearing effects of intranasal IFNγ in RSV-infected adult BALB/c mice demonstrating delayed production of endogenous IFNγ. The dataset is made publicly available to extrapolate the role of IFNγ in RSV-infected adult mice.

Keywords: Cytokine; IFNγ; Immunology; Mice; RSV.

Fig. 1

The percent change in daily weights compared to baseline (A) and viral clearance over time (B) are shown in RSV-infected adult BALB/c mice treated with intranasal IFNγ or PBS. A one-way ANOVA indicates significant differences between the groups; *p< 0.05.

Fig. 2

Cytokine production in the BALF (A-E) of adult BALB/c mice infected with RSV are shown for groups treated with intranasal IFNγ or PBS. Mean and SEM are reported with ≥ 5 mice per group; a one-way ANOVA describes group differences: *p<0.05; **p<0.01.

Fig. 3

The expression of CD68+ macrophages (A-C), CD86+ CD68+ macrophages (D-F), and CD11c+ MHCIIhi dendritic cells (G-I) are reported as percent of large cells in the BALF (A, D, G), total cells in the BALF (B, E, H), and total cells in digested whole lung tissue (C, F, I) following treatment with IFNγ or PBS. Mean and SEM are reported with ≥ 5 mice per group; a one-way ANOVA describes group differences: *p<0.05; ***p<0.001.

Fig. 4

Total CD4 (A) and CD8 (C) T cells and total activated (CD69+) CD4 (B) and CD8 (D) T cells are reported for each group in digested lung tissue (lung) at 6 and 8 dpi in RSV-infected adult mice following treatment with intranasal IFNγ or PBS. No significant differences were determined between groups using a one-way ANOVA.

Fig. 5

Total DX5+ NK cells (A) and activated (CD69+) NK cells (B) in digested lung tissue (lung) are reported in RSV-infected adult mice treated with intranasal IFNγ or PBS. Mean and SEM are reported with ≥ 5 mice per group; a one-way ANOVA describes group differences: **p<0.01.