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. 2017 Jul;63(4):334-340.
doi: 10.18097/PBMC20176304334.

[Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV]

[Article in Russian]
J A Bespyatykh  1 , O A Manicheva  2 , A V Smolyakov  3 , M Z Dogonadze  2 , V Yu Zhuravlev  2 , E A Shitikov  1 , E N Ilina  1
Affiliations

[Influence of cultivation conditions on the proteomic profile of Mycobacterium tuberculosis H37RV]

[Article in Russian]
J A Bespyatykh et al. Biomed Khim. 2017 Jul.

Abstract
in English, Russian

Comparative proteomic profiling of M. tuberculosis H37Rv strains cultured on two different nutrient media, Levenstein-Jensen and Middlebrook 7H11, was performed using a label-free LC-MS/MS approach. It was shown that results obtained from two media possessed high convergence. The only difference was observed in the representation of fumarate reductase FrdB, its abundance was higher in the mycobacterial cells cultured on Levenstein-Jensen medium. The correlation analysis of biological repeats revealed the high convergence of the results obtained from Middlebrook 7H11 medium. Thus, we can conclude that the use of the Middlebrook 7H11 medium is most appropriate in the scientific laboratory.

S ispol'zovaniem bezmetochnogo LC-MS/MS podkhoda provedeno sravnitel'noe proteomnoe profilirovanie Micobacterium tuberculosis H37Rv, kul'tivirovannogo na dvukh raznykh pitatel'nykh sredakh: Levenshteĭna-Ĭensena i Middlbruk 7H11. Pokazano, chto rezul'taty, poluchaemye na dvukh sredakh, obladaiut vysokoĭ skhodimost'iu. Dostovernoe razlichie vyiavleno v predstavlennosti lish' dlia odnogo belka – fumaratreduktazy FrdB, predstavlennost' kotoroĭ byla vyshe v kletkakh mikobakteriĭ, kul'tivirovannykh na srede Levenshteĭna-Ĭensena. Analiz korreliatsii biologicheskikh povtorov vyiavil naibol'shuiu skhodimost' na srede Middlbruk 7N11. Takim obrazom, poluchennye rezul'taty pozvoliaiut sdelat' vyvod, chto ispol'zovanie sredy Middlbruk 7N11 naibolee tselesoobrazno v laboratornykh usloviiakh.

Keywords: Mycobacterium tuberculosis; cultivation conditions; proteins; proteomics; tuberculosis.

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    Bespyatykh J, Shitikov E, Guliaev A, Smolyakov A, Klimina K, Veselovsky V, Malakhova M, Arapidi G, Dogonadze M, Manicheva O, Bespiatykh D, Mokrousov I, Zhuravlev V, Ilina E, Govorun V. Bespyatykh J, et al. Sci Rep. 2019 Dec 17;9(1):19255. doi: 10.1038/s41598-019-55896-z. Sci Rep. 2019. PMID: 31848428 Free PMC article.

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