Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Sep;32(18):2233-2237.
doi: 10.1080/14786419.2017.1371152. Epub 2017 Sep 5.

Optimisation of extraction conditions for total saponins from Cynanchum wallichii using response surface methodology and its anti-tumour effects

Kun-Jun Mao  1 , Ping Huang  1 , Li- Zhang  1 , Hai-Sheng Yu  1 , Xing-Zhuo Zhou  2 , Xi-Yong Ye  1
Affiliations

Optimisation of extraction conditions for total saponins from Cynanchum wallichii using response surface methodology and its anti-tumour effects

Kun-Jun Mao et al. Nat Prod Res. 2018 Sep.

Abstract

Cynanchum wallichii Wight, is a traditional Chinese medicine herb, which is rich in saponins and has varieties of pharmacological activities. In this study, a standardized C. wallichii extract was established and the anti-tumor activity of the total saponins was evaluated by MTT assay. The extraction conditions of the standardized extract was optimized using response surface methodology. The experimental value was in good agreement (the yield 4.28%) with predicted values. The total saponins of the extract showed significant anti-tumor activity against three human tumor cell lines (A549, HepG2 and MCF-7), especially for MCF-7 (IC50. 67.63 μg/mL) cells in vitro.

Keywords: Cynanchum wallichii; anti-tumor; response surface methodology; total saponins.

PubMed Disclaimer

MeSH terms

LinkOut - more resources