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. 2017 Sep 1;54(5):1403-1409.
doi: 10.1093/jme/tjx120.

Arboviruses Isolated From Mosquitoes Collected in Uganda, 2008-2012

Eric C Mossel  1 , Mary B Crabtree  1 , John-Paul Mutebi  1 , Julius J Lutwama  2 , Erin M Borland  1 , Ann M Powers  1 , Barry R Miller  1
Affiliations

Arboviruses Isolated From Mosquitoes Collected in Uganda, 2008-2012

Eric C Mossel et al. J Med Entomol. .

Abstract

A large number of arthropod-borne viruses are endemic to East Africa. As a part of the process of undertaking a systematic characterization of the mosquito fauna of Uganda, we examined mosquitoes collected from 2008 through early 2012 for known and novel viruses. In all, 8,288 mosquito pools containing 157,554 mosquitoes were tested. Twenty-nine isolations of 11 different viruses were made from mosquitoes of nine distinct species and from pools identified only to genus Culex. Identified viruses were from family Togaviridae, alphaviruses Sindbis and Babanki viruses; family Rhabdoviridae, hapaviruses Mossuril and Kamese viruses; family Flaviviridae, flaviviruses West Nile and Usutu viruses; family Phenuiviridae, phlebovirus Arumowot virus; and family Peribunyaviridae, orthobunyaviruses Witwatersrand, Pongola, and Germiston viruses. In addition, a novel orthobunyavirus, provisionally named Mburo virus, was isolated from Coquillettidia metallica (Theobald). This is the first report of Babanki, Arumowot, and Mossuril virus isolation from Uganda.

Keywords: Uganda; arbovirus; mosquito.

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Figures

Fig. 1.
Fig. 1.
Map of Uganda showing mosquito collection sites. Collection sites (•) as well as cities and other locales (○) are indicated. Study site numbers correspond to Table 2.
Fig. 2.
Fig. 2.
Amino acid sequence-based phylogenies of orthobunyavirus nucleocapsid and RNA-dependent RNA polymerase. Full-length amino acid sequences of nucleocapsid (A) and RNA-dependent RNA polymerase (B) were obtained from GenBank and aligned with the derived sequences of Mburo virus (highlighted; GenBank MF074140 and MF074141, respectively). Phylogeny was determined by maximum likelihood analysis using the LG + G + I + F model. The trees were rooted using three members of the Herbevirus genus, family Peribunyaviridae (Marklewitz et al. 2013). Serogroups are shown at the right for reference.
See this image and copyright information in PMC

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