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. 2018 Apr;12(1):15-22.
doi: 10.1007/s12104-017-9772-5. Epub 2017 Sep 6.

Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2

Edith Buchinger  1 , Siv Å Wiik  1   2 , Anna Kusnierczyk  3 , Renana Rabe  3 , Per A Aas  3 , Bodil Kavli  3 , Geir Slupphaug  3 , Finn L Aachmann  4
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Backbone 1H, 13C and 15N chemical shift assignment of full-length human uracil DNA glycosylase UNG2

Edith Buchinger et al. Biomol NMR Assign. 2018 Apr.

Abstract

Human uracil N-glycosylase isoform 2-UNG2 consists of an N-terminal intrinsically disordered regulatory domain (UNG2 residues 1-92, 9.3 kDa) and a C-terminal structured catalytic domain (UNG2 residues 93-313, 25.1 kDa). Here, we report the backbone 1H, 13C, and 15N chemical shift assignment as well as secondary structure analysis of the N-and C-terminal domains of UNG2 representing the full-length UNG2 protein.

Keywords: DNA repair; Intrinsically disordered domain; UNG2; Uracil N-glycosylase isoform 2; Uracil-DNA glycosylase.

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References

    1. Assefa NG, Niiranen L, Willassen NP, Smalås A, Moe E (2012) Thermal unfolding studies of cold adapted uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua). A comparative study with human UNG comparative biochemistry and physiology part. Biochem Mol Biol 161:60–68. doi: 10.1016/j.cbpb.2011.09.007 - DOI
    1. Blatny JM, Brautaset T, WintherLarsen HC, Karunakaran P, Valla S (1997) Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria. Plasmid 38:35–51 - DOI
    1. Hagen L et al (2008) Cell cycle-specific UNG2 phosphorylations regulate protein turnover, activity and association with RPA. EMBO J 27:51–61. doi: 10.1038/sj.emboj.7601958 - DOI
    1. Kavli B et al (2002) hUNG2 Is the Major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup. J Biol Chem 277:39926–39936. doi: 10.1074/jbc.M207107200 - DOI
    1. Keller R (2004) Optimizing the process of nuclear magnetic resonance spectrum analysis and computer aided resonance. Assignment CANTINA Verlag, Zürich

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