Expression and isolation of recombinant tau

Rebecca L Best¹, Peter J Chung², Sarah J Benbow¹, April Savage³, Nichole E LaPointe⁴, Cyrus R Safinya², Stuart C Feinstein⁵

Affiliations

¹ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States.
² University of California, Santa Barbara, Santa Barbara, CA, United States.
³ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁴ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁵ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States. Electronic address: feinstei@lifesci.ucsb.edu.

PMID: 28882309
DOI: 10.1016/bs.mcb.2017.06.001

Expression and isolation of recombinant tau

Rebecca L Best et al. Methods Cell Biol. 2017.

. 2017:141:3-26.

doi: 10.1016/bs.mcb.2017.06.001. Epub 2017 Jul 24.

Authors

Rebecca L Best¹, Peter J Chung², Sarah J Benbow¹, April Savage³, Nichole E LaPointe⁴, Cyrus R Safinya², Stuart C Feinstein⁵

Affiliations

¹ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States.
² University of California, Santa Barbara, Santa Barbara, CA, United States.
³ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁴ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁵ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States. Electronic address: feinstei@lifesci.ucsb.edu.

PMID: 28882309
DOI: 10.1016/bs.mcb.2017.06.001

Abstract

In this chapter, we describe methods for the purification of both untagged and polyhistidine-tagged tau protein. These protocols utilize a bacterial expression system to produce the tau isoform of interest, followed by heat treatment and column chromatography to separate tau from impurities. These techniques yield a biochemically pure protein with which to pursue any number of questions regarding the mechanisms of tau action.

Keywords: Chromatography; Recombinant tau; Tau expression; Tau isolation; Tau purification.

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R01 NS035010/NS/NINDS NIH HHS/United States

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Expression and isolation of recombinant tau

Affiliations

Expression and isolation of recombinant tau

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources