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. 2017 Sep 7;7(1):10867.
doi: 10.1038/s41598-017-11284-z.

Tissue alkalosis in cold-ischemia time

V Denninghoff  1   2 , E H R Olivieri  3   4 , C Fresno  5 , A Uceda  6 , L Mota  4 , A P Suenaga  4 , D M Carraro  3   4 , V R Martins  3 , A Avagnina  6 , F A Soares  7 , A H J Fróes Marques Campos  8   9
Affiliations

Tissue alkalosis in cold-ischemia time

V Denninghoff et al. Sci Rep. .

Abstract

The control of pre-analytical-factors in human biospecimens collected for health research is currently required. Only two previous reports using post-mortem brain samples have tried to address the impact of cold-ischemia on tissue pH. Here we report pH variations according to time (third-order polynomial model) in mice for liver, kidney and lung samples. Tissue alkalosis in cold-ischemia time may be an underlying mechanism of gene expression changes. Therefore, tissue-pH regulation after organ removal may minimize biological stress in human tissue samples.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
pH variation  according to ischemia time evolution in three different organs. (A) Organs third-degree adjusted time polynomial linear mixed model results (dashed lines). (B) Fisher’s least significant difference between organs mean at each data point. Superscript letters indicate significant difference (p < 0.05) using Bonferroni adjusted p-values. In both panels results are presented as mean ± standard error estimation.
See this image and copyright information in PMC

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