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. 2017 Sep 11;22(9):1460.
doi: 10.3390/molecules22091460.

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

Gholamhossein Ebrahimipour  1 , Hossein Sadeghi  2 , Mina Zarinviarsagh  3
Affiliations

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

Gholamhossein Ebrahimipour et al. Molecules. .

Abstract

The Plackett-Burman design and the Box-Behnken design, statistical methodologies, were employed for the optimization lipase and biosurfactant production by Ochrobactrum intermedium strain MZV101 in an identical broth medium for detergent applications. Environmental factor pH determined to be most mutual significant variables on production. A high concentration of molasses at high temperature and pH has a negative effect on lipase and biosurfactant production by O. intermedium strain MZV101. The chosen mathematical method of medium optimization was sufficient for improving the industrial production of lipase and biosurfactant by bacteria, which were respectively increased 3.46- and 1.89-fold. The duration of maximum production became 24 h shorter, so it was fast and cost-saving. In conclusion, lipase and biosurfactant production by O. intermedium strain MZV101 in an identical culture medium at pH 10.5-11 and 50-60 °C, with 1 g/L of molasses, seemed to be economical, fast, and effective for the enhancement of yield percentage for use in detergent applications.

Keywords: Ochrobactrum intermedium strain MZV101; biosurfactant; detergent; lipase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Pareto charts (a) lipase and (b) biosurfactant production by O. intermedium strain MZV101. This chart based on the observations of the Plackett–Burman experimental design for evaluating different variables on lipase and biosurfactant production by the above-mentioned strain, where A is pH, B is temperature, C is molasses in both figures, and D is MgSO₄. The orange color indicates a positive effect, while the blue color indicates a negative effect on the lipase and biosurfactant.
Figure 2
Figure 2
Three-dimensional (3D) response surface plots and their interaction effect between variables for lipase and biosurfactant activity by O. intermedium strain MZV101. (a,d) Interactions between pH and temperature, (b,e) interactions between molasses and pH, and (c,f) interactions between molasses and temperature in basal media with 10 g/L of olive oil with lipases and biosurfactants stimulated by the above-mentioned bacteria.
Figure 3
Figure 3
Lipase enzyme and biosurfactant production in un-optimized and optimized basal medium by O. intermedium strain MZV101. Results were represented as mean ± S.D. All tests are repeated in at least three independent experiments. Error bars indicated standard deviations as shown in this figure.
See this image and copyright information in PMC

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