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. 2017 Jul;11(7):DC32-DC36.
doi: 10.7860/JCDR/2017/24810.10277. Epub 2017 Jul 1.

Low Rate of babA2 Genotype among Iranian Helicobacter pylori Clinical Isolates

Maryam Sohrabi  1 , Reza Khashei  2 , Mahvash Alizadeh  3 , Mohammad-Kazem Hosseini Asl  4 , Mohammad-Ali Nejati  3 , Mahintaj Dara  5 , Abdollah Bazargani  6
Affiliations

Low Rate of babA2 Genotype among Iranian Helicobacter pylori Clinical Isolates

Maryam Sohrabi et al. J Clin Diagn Res. 2017 Jul.

Abstract

Introduction: The Blood Group Antigen-Binding Adhesion (babA), Outer Inflammatory Protein (oipA) and Sialic Acid-Binding Adhesin (sabA) as outer membrane proteins involved in Helicobacter pylori adherence to gastric mucosa have been suggested to have a role in the pathogenesis.

Aim: To investigate the frequency of H. pylori isolates babA2, oipA and sabA genes in Iranian dyspeptic patients.

Materials and methods: DNAs were extracted from H. pylori -positive cultures taken from 100 different dyspeptic patients. Genotyping was performed by Polymerase Chain Reaction (PCR), using the specific primers for babA2, oipA and sabA genes. Chi square test was used to investigate association between variables, p<0.05 was considered statistically significant.

Results: All (100%) isolates possessed oipA and sabA genotypes, whereas babA2 was detected in 22% of isolates. There was no significant relationship between presence of genes with clinical outcome. The combined genotype oipA +/sabA +/ babA2- was correlated with gastritis. The rate of babA2 genotype in our isolates was lower than other Iranian reports.

Conclusion: Frequency of babA2 genotype among H. pylori isolates from Southwest of Iran is considerably less than other regions of Iran. Due to heterogeneity of H. pylori strains in different geographic regions, further work will be needed to understand the role of these virulence genes in H. pylori pathogenesis and their possible association with disease outcome.

Keywords: Gastroduodenal diseases; Virulence genes; oipA; sabA.

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Figures

[Table/Fig-2]:
[Table/Fig-2]:
Agarose gel electrophoresis of glmM, oipA, sabA and babA2 genes in H. pylori isolates. A: M=DNA ladder (100 bp), C+ is positive control (H. pylori ATCC 26695, glmM= 294 bp), C- is negative control (DNase-free water), lanes 1-5 (positive samples). B: M=DNA ladder (100 bp), lanes 1-6 (positive samples of oipA=401 bp). C: M=DNA ladder (100 bp), lanes 1-6 (positive samples of sabA =364 bp). D: M=DNA ladder (100 bp), lane 1 (positive sample of babA2 =832 bp) and lane 2 (negative sample).
See this image and copyright information in PMC

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