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. 2017 Jul;11(7):EC12-EC16.
doi: 10.7860/JCDR/2017/26537.10230. Epub 2017 Jul 1.

Immunohistochemical Study of MUC1 and MUC5AC Expression in Gall Bladder Lesions

Amit Bhoge  1 , Siddhi Gaurish Sinai Khandeparkar  2 , Avinash R Joshi  3 , Bageshri Gogate  3 , Maithili Mandar Kulkarni  2 , Pallavi Bhayekar  4
Affiliations

Immunohistochemical Study of MUC1 and MUC5AC Expression in Gall Bladder Lesions

Amit Bhoge et al. J Clin Diagn Res. 2017 Jul.

Abstract

Introduction: Immunohistochemical (IHC) markers of mucin family are associated with various Gallbladder Lesions (GBLs).

Aim: To study the distribution of GBL with respect to age and sex as well as to analyse the IHC profile of MUC1 and MUC5AC in GBLs and attempt correlation with clinical and histopathological findings.

Materials and methods: The present study was conducted over a period of six years. A technique of manual tissue array was employed for cases subjected to IHC using MUC1 and MUC5AC. Results were statistically analysed using software program "The Primer of Biostatistics 5.0".

Results: A total of 629 GBL were encountered. Out of 605 of non-neoplastic lesions, 32 (5.29%) expressed MUC1 while 515 (85.12%) cases expressed MUC5AC. Out of 24 cases of neoplastic GBL, 20 cases (83.33%) showed positivity for MUC1 and 9 cases (37.5%) were positive for MUC5AC. The rate of MUC1 expression was significantly higher in Gall Bladder Cancer (GBC) {18GB carcinoma (ca) +3 Carcinoma In Situ (CIS)} (85.71%) than chronic cholecystitis (4.71%). The positive rate of MUC5AC expression was significantly lower in GBC (28.57%) than chronic cholecystitis (87.19%). The percentage of cases showing MUC1 expression increased as the severity of disease progressed from hyperplasia to CIS. The percentage of cases showing MUC5AC expression decreased as the severity of disease progressed from hyperplasia to CIS.

Conclusion: In this study, 96.18% cases were non neoplastic GBL of which chronic cholecystitis (87.77%) was predominant. 3.81% of the GBL constituted for neoplastic lesions of which 75% were GBC. MUC1 showed higher rates of expression in neoplastic GBL. MUC5AC showed higher rates of expression in non neoplastic GBL. Expression of MUC1 and MUC5AC might be closely related to pathogenesis of neoplastic and non neoplastic GBL.

Keywords: Dysplasia; Gall bladder carcinoma; Metaplasia; Mucin markers.

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Figures

[Table/Fig-8]:
[Table/Fig-8]:
MUC1 expression in GBL: a) Hyperplasia showing +1 positivity (40X); b) Metaplasia showing +1 positivity (40X); c) Dysplasia showing +2 positivity (10X); d) CIS showing +3 positivity (40X); e) Invasive carcinoma showing +3 positivity (40X). Arrow denotes the +1 and +2 positivity in the same photo photomicrograph.
[Table/Fig-9]:
[Table/Fig-9]:
MUC5AC expression in GBL: a) Hyperplasia showing +2 positivity (10X); b) Metaplasia showing +3 positivity (40X); c) Dysplasia showing +1 positivity (40X); d) Invasive carcinoma showing +1 positivity (40X).
See this image and copyright information in PMC

References

    1. Mohan H, Punia RPS, Dhawan SB, Ahal S, Sekhon MS. Original Morphological spectrum of gallstone disease in 1100 cholecystectomies in North India. Indian J Surg. 2005;67:140–42.
    1. Kapoor VK, McMichael AJ. Gallbladder cancer: An ‘Indian’ disease. Natl Med J India. 2003;16:209–13. - PubMed
    1. Barreto SG, Dutt A, Chaudhary A. A genetic model for gallbladder carcinogenesis and its dissemination. Annals of Oncology. 2014;25:1086–97. - PMC - PubMed
    1. Vilkin A, Nudelman I, Morgenstern S, Geller A, Bar Dayan Y, Levi Z, et al. Gallbladder inflammation is associated with increase in mucin expression and pigmented stone formation. Dig Dis Sci. 2007;52:1613–20. - PubMed
    1. Xiong L, Yang Z, Yang L, Liu J, Miao X. Expressive levels of MUC1 and MUC5AC and their clinicopathologic significances in the benign and malignant lesions of gallbladder. J Surg Oncol. 2012;105:97–103. - PubMed

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