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. 2017:2017:3196924.
doi: 10.1155/2017/3196924. Epub 2017 Aug 15.

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

Nayana Gunathilaka  1 , Tharaka Ranathunge  2 , Lahiru Udayanga  2 , Wimaladharma Abeyewickreme  3
Affiliations

Efficacy of Blood Sources and Artificial Blood Feeding Methods in Rearing of Aedes aegypti (Diptera: Culicidae) for Sterile Insect Technique and Incompatible Insect Technique Approaches in Sri Lanka

Nayana Gunathilaka et al. Biomed Res Int. 2017.

Abstract

Introduction: Selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors.

Methodology: Artificial membrane feeding techniques, namely, glass plate, metal plate, and Hemotek membrane feeding method, and three blood sources (human, cattle, and chicken) were evaluated based on feeding rates, fecundity, and hatching rates of Aedes aegypti. Significance in the variations among blood feeding was investigated by one-way ANOVA, cluster analysis of variance (ANOSIM), and principal coordinates (PCO) analysis.

Results: Feeding rates of Ae. aegypti significantly differed among the membrane feeding techniques as suggested by one-way ANOVA (p < 0.05). The metal plate method was identified as the most efficient and cost-effective feeding technique. Blood feeding rate of Ae. aegypti was higher with human blood followed by cattle and chicken blood, respectively. However, no significant difference was observed from the mosquitoes fed with cattle and human blood, in terms of fecundity, oviposition rate, and fertility as suggested by one-way ANOVA (p > 0.05).

Conclusions: Metal plate method could be recommended as the most effective membrane feeding technique for mass rearing of Ae. aegypti, due to its high feeding rate and cost effectiveness. Cattle blood could be recommended for mass rearing Ae. aegypti.

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Figures

Figure 1
Figure 1
Setup of the glass plate method.
Figure 2
Figure 2
Metal plate and the setup of the metal plate method.
Figure 3
Figure 3
Setup of the Hemotek membrane feeding system.
Figure 4
Figure 4
Dendrogram for the cluster analysis of blood source success by Ae. aegypti.
Figure 5
Figure 5
Ordination of the different blood sources on PCO1 and PCO2 scores of PCO analysis in terms of success of Ae. aegypti.
See this image and copyright information in PMC

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