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. 2017 Jun;58(2):E166-E172.

Five-year microbiological monitoring of wards and operating theatres in southern Italy

V La Fauci  1 , C Genovese  2 , A Facciolà  2 , M A R Palamara  2 , R Squeri  1
Affiliations

Five-year microbiological monitoring of wards and operating theatres in southern Italy

V La Fauci et al. J Prev Med Hyg. 2017 Jun.

Abstract

Introduction: Nosocomial infections are one of the greatest problems in public health. Several studies have highlighted the role played by the hospital environment as a possible source of transmission of nosocomial pathogens.

Methods: A five-year monitoring of bacterial contamination on healthcare workers hands, surfaces most closely in contact with inpatient wards, operating theatres and "at rest" and "in use" operating theatre air samples. For the samples, we used sterile swabs, contact slides, manual API, and automated VITEK systems for identification.

Results: In the five-year period, a total of 9396 samples were collected and analysed. In ward patients, 4398 samplings were carried out with 4.7%, 9.4%, 7%, 10.8% and 7.9% positive results respectively from 2010 to 2014. For hands, 648 samplings were carried out, with a positivity of 40.74%. In operating theatres, 4188 samples were taken, with a positivity of 11.9%. Regarding air in empty and full theatres, 1962 samplings were carried out with a positivity rate equal to 31.9%. The monitoring showed a low rate of contamination with a progressive decrease in the fiveyear period on operating theatres surfaces and hands, while there was an increase in the surgical site wards and in the air of operating rooms.

Conclusions: Our investigation has revealed the presence of pathogens on the assessed surfaces and the need for environmental monitoring, which can be a valuable tool for reducing contamination.

Keywords: HCAI; Microbiological surveillance; Operating rooms; Wards.

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Conflict of interest statement

the authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Percentage of microorganisms by type in the 5 years in the wards.
Fig. 2.
Fig. 2.
Temporal trend in the five years of positivity for operating units.
Fig. 3.
Fig. 3.
Percentage of microorganisms by type in the period on the hands of health care workers.
Fig. 4.
Fig. 4.
Percentage of microorganisms by type in the five years in operating rooms.
Fig. 5.
Fig. 5.
Time course in the five years of positive operating rooms.
See this image and copyright information in PMC

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