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Review
. 2017 Dec;55(12):3339-3349.
doi: 10.1128/JCM.00707-17. Epub 2017 Sep 13.

Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue

Junxiong Pang  1   2 , Po Ying Chia  3 , David C Lye  3   4   5 , Yee Sin Leo  3   2   4   5
Affiliations
Review

Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue

Junxiong Pang et al. J Clin Microbiol. 2017 Dec.

Abstract

Dengue detection strategies involve viral RNA, antigen, and/or antibody detection. Each strategy has its advantages and disadvantages. Optimal, user-friendly, rapid diagnostic tests based on immunochromatographic assays are pragmatic point-of-care tests (POCTs) in regions where dengue is endemic where there are limited laboratory capabilities and optimal storage conditions. Increasingly, there is a greater public health significance for a multiplexing assay that differentiates dengue from Zika or pathogens with similar clinical presentations. Although there have been many assay/platform developments toward POCTs, independent validation and implementation remain very limited. This review highlights the current key progress and challenges toward the development of a dengue POCT.

Keywords: biotechnology; clinical diagnosis; dengue fever; diagnostics tools; flavivirus; neutralizing antibodies; point-of-care test; serology; viral clearance.

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Figures

FIG 1
FIG 1
Schematic profiles of dengue viremia, NS-1, and anti-dengue IgM and IgG in blood during primary and secondary dengue infections over time.
See this image and copyright information in PMC

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