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. 2015 Dec;23(4):652-659.
doi: 10.1016/j.jfda.2015.03.006. Epub 2015 May 19.

A rapid and sensitive spectrophotometric method for the determination of benzoyl peroxide in wheat flour samples

Kraingkrai Ponhong  1 , Sam-Ang Supharoek  2 , Watsaka Siriangkhawut  1 , Kate Grudpan  3
Affiliations

A rapid and sensitive spectrophotometric method for the determination of benzoyl peroxide in wheat flour samples

Kraingkrai Ponhong et al. J Food Drug Anal. 2015 Dec.

Abstract

A simple, rapid, and sensitive spectrophotometric method for the determination of benzoyl peroxide (BPO) in wheat flour samples was developed. The detection principle is based on BPO reacted with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to obtain a blue-green colored product that was detected at 415 nm by spectrophotometry. The effect of factors influencing the color reaction was investigated. Under the selected conditions, the linear range for quantification of BPO was observed between 0.2-1.0 mg L-1 with r2 = 0.998. The limit of detection (LOD) was 0.025 mg L-1. The developed method obtained superior precision (relative standard deviation < 2%) using 11 repeatability at 0.2 mg L-1, 0.6 mg L-1, and 0.8 mg L-1. The proposed methodology was successfully applied to determine BPO in wheat flour samples.

Keywords: ABTS; benzoyl peroxide; colorimetric reaction; wheat flour.

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Conflict of interest statement

Conflicts of interest

All authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
The possibility reaction of the proposed system.
Fig. 2
Fig. 2
Absorption spectra of 1.0 mg L−1 BPO (dotted line), ABTS 10 mg L−1 (broken line) and the blue-green color solution after mixed 1.0 mg L−1 BPO with 10 mg L−1 ABTS (unbroken line). ABTS = 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); BPO = benzoyl peroxide.
Fig. 3
Fig. 3
Effects of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) concentration.
Fig. 4
Fig. 4
Calibration curve graph of the developed method.
Fig. 5
Fig. 5
Chromatograms of (A) sample No. 6 without spiked benzoyl peroxide (BPO) standard, (B) BPO standard 50 μg g−1 and (C) sample No. 6 with spiked BPO 90 μg g−1.
See this image and copyright information in PMC

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