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. 2017 Jul;25(3):654-666.
doi: 10.1016/j.jfda.2016.11.017. Epub 2017 Feb 14.

Microencapsulation of fish oil using supercritical antisolvent process

Fahim Tamzeedul Karim  1 , Kashif Ghafoor  2 , Sahena Ferdosh  3 , Fahad Al-Juhaimi  2 , Eaqub Ali  4 , Kamaruzzaman Bin Yunus  3 , Mir Hoseini Hamed  5 , Ashraful Islam  6 , Mohammad Asif  7 , Mohammed Zaidul Islam Sarker  1
Affiliations

Microencapsulation of fish oil using supercritical antisolvent process

Fahim Tamzeedul Karim et al. J Food Drug Anal. 2017 Jul.

Abstract

In order to improve the encapsulation process, a newly supercritical antisolvent process was developed to encapsulate fish oil using hydroxypropyl methyl cellulose as a polymer. Three factors, namely, temperature, pressure, and feed emulsion rate were optimized using response surface methodology. The suitability of the model for predicting the optimum response value was evaluated at the conditions of temperature at 60°C, pressure at 150 bar, and feed rate at 1.36 mL/min. At the optimum conditions, particle size of 58.35 μm was obtained. The surface morphology of the micronized fish oil was also evaluated using field emission scanning electron microscopy where it showed that particles formed spherical structures with no internal voids. Moreover, in vitro release of oil showed that there are significant differences of release percentage of oil between the formulations and the results proved that there was a significant decrease in the in vitro release of oil from the powder when the polymer concentration was high.

Keywords: HPMC; microencapsulation; omega 3; supercritical antisolvent.

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Conflict of interest statement

Conflict of interest

All contributing authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic diagram of supercritical antisolvent (SAS) process (adapted from Ref. [5]).
Figure 2
Figure 2
Schematic diagram of supercritical antisolvent (SAS) system (adapted from Ref. [19]).
Figure 3
Figure 3
Particle collector used in supercritical antisolvent (SAS) system (Adapted from Ref. [19]).
Figure 4
Figure 4
Response optimizer at the optimum condition for target goal.
Figure 5
Figure 5
Response contour plot of particle size (μm) at a feasible optimum condition.
Figure 6
Figure 6
Response surface plot of particle size (μm) at a feasible optimum condition.
Figure 7
Figure 7
Effect of total solid content on encapsulation efficiency.
Figure 8
Figure 8
Morphology of fish oil powder (AF1, AF2, AF3, and AF4) at different concentration.
Figure 9
Figure 9
Effect of storage time on the peroxide value of formulation AF1, AF2, AF3, AF4.
See this image and copyright information in PMC

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