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. 2017 Jul 8;8(34):55950-55957.
doi: 10.18632/oncotarget.19116. eCollection 2017 Aug 22.

PKCθ utility in diagnosing c-KIT/DOG-1 double negative gastrointestinal stromal tumors

Attila Kövecsi #  1   2 , Ioan Jung #  1 , Zoltan Szentirmay  3 , Tivadar Bara  4 , Tivadar Bara Jr  4 , Daniel Popa  4 , Simona Gurzu  1   2   5
Affiliations

PKCθ utility in diagnosing c-KIT/DOG-1 double negative gastrointestinal stromal tumors

Attila Kövecsi et al. Oncotarget. .

Abstract

Background: The aim of this study was to evaluate the diagnosis value of an immunohistochemical (IHC) panel of three antibodies for the diagnosis of gastrointestinal stromal tumors (GISTs).

Material and methods: In 80 consecutive GISTs without lymph node metastases, the IHC examinations were performed using the antibodies CD117 (c-KIT), DOG-1 and c-theta (PKCθ) protein. The diagnostic value of PKCθ in c-KIT/DOG-1 negative GISTs has been explored in fewer than 10 Medline-indexed papers.

Results: The c-KIT, PKCθ and DOG-1 positivity was noted in 92.50% (n = 74), 90% (n = 72) and 76.25% (n = 61) of the cases, respectively. All of the C-KIT negative cases (n = 6) were also DOG-1 negative but displayed PKCθ positivity. All of the DOG-1 positive cases (n = 61) also expressed c-KIT. No correlation between the examined markers and clinicopathological parameters was noted.

Conclusions: The PKCθ sensitivity is similar to c-KIT and superior to DOG-1 sensitivity. All of the c-KIT/DOG-1 negative GISTs seem to express PKCθ. For a proper diagnosis of GIST, the c-KIT/DOG-1/PKCθ panel should be used, with possible therapeutic but not prognostic value.

Keywords: CD117; GIST; Pathology Section; c-theta; diagnosis; protein kinase.

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Conflict of interest statement

CONFLICT OF INTERESTS No conflict of interests are declared.

Figures

Figure 1
Figure 1. Correlation between c-KIT, DOG-1 and PKCθ expression revealed by Venn-diagram
Figure 2
Figure 2
Negative immunoexpression of c-KIT (A), DOG-1 (B) and PKCθ (C) in gastrointestinal stromal tumors.
Figure 3
Figure 3
Positive immunoexpression of c-KIT (A), DOG-1 (B) and PKCθ (C) in gastrointestinal stromal tumors.
See this image and copyright information in PMC

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