DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method
- PMID: 28916151
- DOI: 10.1016/j.biochi.2017.09.007
DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method
Abstract
A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence. This novel method provides stabilized high-affinity DNA aptamers for diagnostic and therapeutic applications.
Keywords: Aptamer; ExSELEX; Genetic alphabet expansion; Mini-hairpin DNA; Unnatural base pair.
Copyright © 2017 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.
Similar articles
-
Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165 toward pharmaceutical applications.Nucleic Acids Res. 2016 Sep 6;44(15):7487-94. doi: 10.1093/nar/gkw619. Epub 2016 Jul 7. Nucleic Acids Res. 2016. PMID: 27387284 Free PMC article.
-
DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.Methods Mol Biol. 2016;1380:47-60. doi: 10.1007/978-1-4939-3197-2_4. Methods Mol Biol. 2016. PMID: 26552815
-
Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications.Sci Rep. 2015 Dec 22;5:18478. doi: 10.1038/srep18478. Sci Rep. 2015. PMID: 26690672 Free PMC article.
-
Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology.Curr Opin Chem Biol. 2018 Oct;46:108-114. doi: 10.1016/j.cbpa.2018.07.017. Epub 2018 Jul 27. Curr Opin Chem Biol. 2018. PMID: 30059833 Review.
-
Methods for Improving Aptamer Binding Affinity.Molecules. 2016 Mar 28;21(4):421. doi: 10.3390/molecules21040421. Molecules. 2016. PMID: 27043498 Free PMC article. Review.
Cited by
-
Functional Nucleic-Acid-Decorated Spherical Nanoparticles: Preparation Strategies and Current Applications in Cancer Therapy.Small Sci. 2021 Feb 9;1(3):2000056. doi: 10.1002/smsc.202000056. eCollection 2021 Mar. Small Sci. 2021. PMID: 40213613 Free PMC article.
-
Programmable manipulation of oligonucleotide-albumin interaction for elongated circulation time.Nucleic Acids Res. 2022 Apr 8;50(6):3083-3095. doi: 10.1093/nar/gkac156. Nucleic Acids Res. 2022. PMID: 35293579 Free PMC article.
-
Prospects and challenges of dynamic DNA nanostructures in biomedical applications.Bone Res. 2022 May 23;10(1):40. doi: 10.1038/s41413-022-00212-1. Bone Res. 2022. PMID: 35606345 Free PMC article. Review.
-
Nucleic Acid-Based Treatments Against COVID-19: Potential Efficacy of Aptamers and siRNAs.Front Microbiol. 2021 Nov 8;12:758948. doi: 10.3389/fmicb.2021.758948. eCollection 2021. Front Microbiol. 2021. PMID: 34858370 Free PMC article. Review.
-
Strict Interactions of Fifth Letters, Hydrophobic Unnatural Bases, in XenoAptamers with Target Proteins.J Am Chem Soc. 2023 Sep 20;145(37):20432-20441. doi: 10.1021/jacs.3c06122. Epub 2023 Sep 7. J Am Chem Soc. 2023. PMID: 37677157 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
