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. 2018:1661:93-101.
doi: 10.1007/978-1-4939-7258-6_7.

In Vitro Translation Assays for Selenocysteine Insertion

Mark H Pinkerton  1 , Paul R Copeland  2
Affiliations

In Vitro Translation Assays for Selenocysteine Insertion

Mark H Pinkerton et al. Methods Mol Biol. 2018.

Abstract

The molecular characterization of the protein and RNA factors that are required for Sec incorporation in mammals has been largely carried out using in vitro translation systems specifically modified for this purpose. This chapter outlines the various systems and modifications that have been used to decipher the mechanism of Sec incorporation.

Keywords: 3′ UTR; Rabbit reticulocyte lysate; SECISBP2; Translation; Wheat germ lysate; eEFSec; mRNA.

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