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. 2017 Mar 6;11(1):143-162.
doi: 10.3897/CompCytogen.. eCollection 2017.

Variability and evolutionary implications of repetitive DNA dynamics in genome of Astyanax scabripinnis (Teleostei, Characidae)

Patrícia Barbosa  1 , Eliza Viola Leal  2 , Maelin da Silva  2 , Mara Cristina de Almeida  2 , Orlando Moreira-Filho  1   2 , Roberto Ferreira Artoni  1   2
Affiliations

Variability and evolutionary implications of repetitive DNA dynamics in genome of Astyanax scabripinnis (Teleostei, Characidae)

Patrícia Barbosa et al. Comp Cytogenet. .

Abstract

DNA sequences of multiple copies help in understanding evolutionary mechanisms, genomic structures and karyotype differentiation. The current study investigates the organization and distribution of different repetitive DNA in the standard complement and B chromosomes in Astyanax scabripinnis (Jenyns, 1842) chromosomes from three allopatric populations in Campos do Jordão region, São Paulo State, Brazil. The location of microsatellite sequences showed different chromosome distribution between Lavrinha Farm Stream (LFS) and Lake of Pedalinho (LP) populations. However, the karyotype of these populations basically followed the pattern of dispersed distribution in the A complement, conspicuous in telomeric/interstitial regions and preferential accumulation in the B chromosome. The B chromosome showed heterogeneous location of microsatellite probes CA, CAC and GA. The H3 and H4 histone genes were isolated from the total genome of the species and then the chromosomal mapping was performed by fluorescence in situ hybridization (FISH). The FISH signals showed high similarity for the probes H3 and H4 mapping in genomes of the populations analyzed. The sequences (GATA) n revealed a sex-specific trend between the chromosomal location in males and females at (LFS) and (LP) populations. Although species that comprise the Astyanax scabripinnis complex do not have morphologically differentiated sex chromosomes, the preferential GATA location - sex-associated - may represent a sex chromosome in differentiation.

Keywords: B chromosomes microsatellites accumulation; Microsatellites chromosomic mapping; sex-specific GATAn sequence location.

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Figures

Figure 1.
Figure 1.
Karyotype of the three Astyanax scabripinnis populations (a, b Lavrinha Farm Stream – LSF); (c, d Lake of Pedalinho – LP); (e, f Ribeirão das Perdizes – RP). In a, c, e are visualized signals of 18S (red) and 5S (green) with rDNA probes and in b, d, f H3 (red) and H4 (green) with histones probes, after double Fluorescence in situ Hybridization (FISH), respectively. Scales bar: 10 µm.
Figure 2.
Figure 2.
Fluorescence in situ Hybridization in metaphases of the three Astyanax scabripinnis populations (a, b Lavrinha Farm Stream – LSF); (c, d Lake of Pedalinho – LP); (e, f Ribeirão das Perdizes – RP) with (GATA)n probe. a, c, e males and b, d, f females. Scales bar: 10 µm
Figure 3.
Figure 3.
B chromosome of Astyanax scabripinnis showing the chromatin and localization of DNA sequences by FISH.
Figure 4.
Figure 4.
Fluorescence in situ Hybridization in metaphases of the three Astyanax scabripinnis populations (a, b, c Lavrinha Farm Stream – LSF); (d, e, f Lake of Pedalinho – LP); (g, h, i Ribeirão das Perdizes - RP) with microsatellites probes. Scales bar: 10 µm.
Figure 5.
Figure 5.
Idiograms of the three Astyanax scabripinnis populations (LFS: Lavrinha Farm Stream); (LP: Lake of Pedalinho); (RP: Ribeirão das Perdizes) highlighting all markers of molecular cytogenetics used in this study.
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