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. 2017 Mar 31;11(2):203-212.
doi: 10.3897/CompCytogen.v11i2.10282. eCollection 2017.

Chromosome mapping in Abracris flavolineata (De Geer, 1773) (Orthoptera) from the Iguaçu National Park - Foz do Iguaçu, Paraná, Brazil

Mariana Bozina Pine  1 , Raquel Bozini Gallo  1 , Carlos Roberto Maximiano da Silva  1 , Larissa Forim Pezenti  1 , Fernando Campos De Domenico  2 , Vilma Loreto  3 , Renata da Rosa  1
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Chromosome mapping in Abracris flavolineata (De Geer, 1773) (Orthoptera) from the Iguaçu National Park - Foz do Iguaçu, Paraná, Brazil

Mariana Bozina Pine et al. Comp Cytogenet. .

Abstract

In this paper, we present the cytomolecular analysis of a population of Abracris flavolineata collected in the largest fragment of the Brazilian Atlantic forest, the Iguaçu National Park. The diploid number in males was 23 (22+X0), with two large pairs (1-2), 7 medium (3-9), 2 small (10-11) and the X chromosome of medium size. Heterochromatic blocks were evident in the pericentromeric regions of all chromosomes. Heterogeneity in the distribution of heterochromatin was observed, with a predominance of DAPI+ blocks. However, some chromosomes showed CMA3+ blocks and other DAPI+/CMA3+ blocks. The 18S rDNA sites were distributed on the short arms of 5 pairs. In two of these pairs, such sites were in the same chromosome bearing 5S rDNA, and one of the bivalents, they were co-located. Histone H3 genes were found on one bivalent. The results added to the existing cytogenetic studies provided evidence of great karyotypic plasticity in the species. This pliancy may be the result of vicariant events related to the geographical distribution of different populations of A. flavolineata.

Keywords: Acrididae; Brazilian Atlantic forest; chromosome banding; fluorescence in situ hybridization; grasshopper.

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Figures

Figure 1.
Figure 1.
Mitotic and meiotic stages of Abracris flavolineata by Giemsa conventional staining (a–c), C-banding (d–f) and fluorochromes staining (g–i): a mitotic metaphase b diakinesis c metaphase II d pachytene e diakinesis f metaphase II g mitotic metaphase by DAPI staining h mitotic metaphase by CMA3 staining i overlapping DAPI/CMA3. The numbers correspond to autosomes. X corresponds to the sex chromosome. The arrows indicate the discrete heterochromatic bands. Bar= 5µm.
Figure 2.
Figure 2.
Fluorescent in situ hybridization of A. flavolineata in meiocytes: a, b 18S rDNA (green) and 5S rDNA (red) probes c, d histone H3 gene probe: a pachytene b–d two cells in metaphase II. The numbers correspond to autosomes. X corresponds to the sex chromosome. The arrows indicate the histone H3 gene sites. Bar= 5µm.
Figure 3.
Figure 3.
Idiogram showing the mapping of different sequences studied in Abracris flavolineata from the Iguaçu National Park.
See this image and copyright information in PMC

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