Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019:1576:301-312.
doi: 10.1007/7651_2017_68.

Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly

Yuqing Zhu  1   2 , Vahid Serpooshan  3 , Sean Wu  3 , Utkan Demirci  4 , Pu Chen  5   6 , Sinan Güven  7   8
Affiliations

Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly

Yuqing Zhu et al. Methods Mol Biol. 2019.

Abstract

There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.

Keywords: 3D organotypic microtissues; Acoustic assembly; Acoustic waves; Cardiac scaffold; Cardiomyocyte; Cell assembly; Cell spheroids; In vitro tissue model; Regenerative medicine; Spheroid assembly; Tissue engineering.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Illustration of acoustic node assembly of organotypic microtissues. Cells/spheroids are first loaded in the assembly chamber. Then standing waves are applied to assemble cells/spheroids into pre-defined patterns based on node and antinode zones on standing waves in a fluidic environment. Further assembled cells/spheroids are immobilized in fibrin hydrogel and maturated in culture media
Fig. 2
Fig. 2
Schematic of experimental setup for acoustic assembly
Fig. 3
Fig. 3
Schematic of optimized chemically defined cardiac differentiation protocol [10]
See this image and copyright information in PMC

References

    1. Guven S et al. (2015) Multiscale assembly for tissue engineering and regenerative medicine. Trends Biotechnol 33(5):269–279 - PMC - PubMed
    1. Esch EW, Bahinski A, Huh D (2015) Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov 14(4):248–260 - PMC - PubMed
    1. Asghar W et al. (2013) In vitro three-dimensional cancer culture models In: Bae YH, Mrsny RJ, Park K (eds) Cancer targeted drug delivery: an elusive dream. Springer, New York, NY, pp 635–665
    1. Arslan-Yildiz A et al. (2016) Towards artificial tissue models: past, present, and future of 3D bioprinting. Biofabrication 8(1):014103. - PubMed
    1. Serpooshan V, Mahmoudi M, Hu DA, Hu JB, Wu SM (2017) Bioengineering cardiac constructs using 3D printing. J 3D Printing Med 1(2):123–139

Publication types

LinkOut - more resources