Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application

Abstract

Background: Alkaline thermostable lipase and biosurfactant producing bacteria are very interested at detergent applications, not only because of their eco-friendly characterize, but alsoproduction lipase and biosurfactant by using cheap materials. Ochrobactrum intermedium strain MZV101 was isolated as washing powder resistant, alkaline thermostable lipase and biosurfactant producing bacterium in order to use at detergent applications.

Methods: O. intermedium strain MZV101 produces was lipase and biosurfactant in the same media with pH 10 and temperature of 60 °C. Washing test and some detergent compatibility character of lipase enzyme and biosurfactant were assayed. The antimicrobial activity evaluated against various bacteria and fungi.

Results: Lipase and biosurfactant produced by O. intermedium strain MZV101 exhibited high stability at pH 10-13 and temperature of 70-90 °C, biosurfactant exhibits good stability at pH 9-13 and thermostability in all range. Both lipase and biosurfactant were found to be stable in the presence of different metal ions, detergents and organic solvents. The lipase enzyme extracted using isopropanol with yield of 69.2% and biosurfactant with ethanol emulsification index value of 70.99% and yield of 9.32 (g/l). The single band protein after through from G-50 Sephadex column on SDS-PAGE was calculated to be 99.42 kDa. Biosurfactant O. intermedium strain MZV101 exhibited good antimicrobial activity against Gram-negative bacteria and against various bacterial pathogens. Based upon washing test biosurfactant and lipase O. intermedium strain MZV101considered being strong oil removal.

Conclusion: The results of this study indicate that isolated lipase and biosurfactant with strong oil removal, antimicrobial activity and good stability could be useful for detergent applications.

Keywords: Biosurfactant; Lipase; Ochrobactrum intermedium; Washing powder.

Fig. 1

Phylogenetic tree. The Phylogenetic tree is generated on the basis of 16S rDNA gene sequence of isolated O. intermedium strain MZV101 using neighbor-joining method. According to 16S rDNA gene sequencing, BLAST in NCBI GenBank (access number KX619441) and strain was closely related to Ochrobactrum intermedium

Fig. 2

Effect of pH on lipase and biosurfactant stability of O. intermedium strain MZV101.The test were studded in pH 5–13 in temperature 37 °C which incubated 4 h for lipase and 24 h for biosurfactant. Results were represented as mean ± S.D. and all tests are examined at least in triplicate independent experiments. Error bar has indicated standard deviations shown in all figures

Fig. 3

Effect of temperature on lipase and biosurfactant stability of O. intermedium strain MZV101. Experiments were measured in temperature 10–90 °C in optimal pH 10 which incubated 4 h for lipase and 24 h for biosurfactant

Fig. 4

SDS-PAGE gel of Lipase enzyme O. intermedium strain MZV101. (A) standard marker proteins, (B) Single protein band become visible at 99.42 kDa on 12% sodium dodecyl sulfate-polyacrylamide gel after stained with coomassie blue G 250