Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Moegi Kuroda¹, Tsuneyuki Masuda¹, Mika Ito², Yuki Naoi³, Yen Hai Doan⁴, Kei Haga⁵, Shinobu Tsuchiaka³, Mai Kishimoto³, Kaori Sano³, Tsutomu Omatsu³, Yukie Katayama³, Mami Oba³, Hiroshi Aoki⁶, Toru Ichimaru⁷, Fujiko Sunaga⁸, Itsuro Mukono², Hiroshi Yamasato¹, Junsuke Shirai³, Kazuhiko Katayama⁵, Tetsuya Mizutani³, Tomoichiro Oka⁹, Makoto Nagai¹⁰

Affiliations

¹ Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori 683-0017, Japan.
² Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
³ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
⁴ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
⁵ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan; Laboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Minato, Tokyo 108-8641, Japan.
⁶ Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
⁷ Department of Health and Medical Sciences, Ishikawa Prefectural Nursing University, Kahoku, Ishikawa 929-1210, Japan.
⁸ Laboratory of Infectious Diseases, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
⁹ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. Electronic address: oka-t@nih.go.jp.
¹⁰ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan. Electronic address: m-nagai@ishikawa-pu.ac.jp.

PMID: 28923281
DOI: 10.1016/j.meegid.2017.09.013

Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Moegi Kuroda et al. Infect Genet Evol. 2017 Nov.

. 2017 Nov:55:209-217.

doi: 10.1016/j.meegid.2017.09.013. Epub 2017 Sep 18.

Authors

Affiliations

¹ Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori 683-0017, Japan.
² Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
³ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
⁴ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
⁵ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan; Laboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Minato, Tokyo 108-8641, Japan.
⁶ Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
⁷ Department of Health and Medical Sciences, Ishikawa Prefectural Nursing University, Kahoku, Ishikawa 929-1210, Japan.
⁸ Laboratory of Infectious Diseases, Azabu University, Sagamihara, Kanagawa 252-5201, Japan.
⁹ Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. Electronic address: oka-t@nih.go.jp.
¹⁰ Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan. Electronic address: m-nagai@ishikawa-pu.ac.jp.

PMID: 28923281
DOI: 10.1016/j.meegid.2017.09.013

Abstract

Sapoviruses (SaV) are enteric viruses infecting humans and animals. SaVs are highly diverse and are divided into multiple genogroups based on structural protein (VP1) sequences. SaVs detected from pigs belong to eight genogroups (GIII, GV, GVI, GVII, GVIII, GIX, GX, and GXI), but little is known about the SaV genogroup distribution in the Japanese pig population. In the present study, 26 nearly complete genome (>6000 nucleotide: nt) and three partial sequences (2429nt, 4364nt, and 4419nt in length, including the entire VP1 coding region) of SaV were obtained from one diarrheic and 15 non-diarrheic porcine feces in Japan via a metagenomics approach. Phylogenetic analysis of the complete VP1 amino acid sequence (aa) revealed that 29 porcine SaVs were classified into seven genogroups; GIII (11 strains), GV (1 strain), GVI (3 strains), GVII (6 strains), GVIII (1 strain), GX (3 strains), and GXI (4 strains). This manuscript presents the first nearly complete genome sequences of GX and GXI, and demonstrates novel intergenogroup recombination events.

Keywords: Genogroup; Japan; Pig; Recombination; Sapovirus.

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Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Affiliations

Genetic diversity and intergenogroup recombination events of sapoviruses detected from feces of pigs in Japan

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical

Research Materials