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. 1988 Jan;42(1):143-59.

Identification of more than 500 RFLPs by screening random genomic clones

J W Schumm  1 , R G Knowlton, J C Braman, D F Barker, D Botstein, G Akots, V A Brown, T C Gravius, C Helms, K Hsiao, et al.
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Identification of more than 500 RFLPs by screening random genomic clones

J W Schumm et al. Am J Hum Genet. 1988 Jan.

Abstract

As part of our genome-mapping effort, we undertook a large-scale screening study to identify RFLPs useful as genetic markers. Some 1,664 single-copy or repeat-containing phage clones from a Charon 4A genomic library were tested for polymorphism against a panel of DNAs, from five unrelated individuals, digested with eight restriction enzymes. Approximately 30% (515) of the clones revealed polymorphism by Southern hybridization; 67 loci detected had PIC values greater than .5. Restriction enzymes MspI, TaqI, and RsaI were most efficient in detecting polymorphism within the 1-20-kb-fragment size range resolved. With only one exception each of the clones detected polymorphism originating from a single locus.

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