Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Aug 24;5(8):apps.1700055.
doi: 10.3732/apps.1700055. eCollection 2017 Aug.

Characterization and transferability of microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Bronwyn M Ayre  1   2 , Janet M Anthony  1   2 , David G Roberts  2   3 , Richard J N Allcock  4   5 , Siegfried L Krauss  1   2
Affiliations

Characterization and transferability of microsatellites for the Kangaroo Paw, Anigozanthos manglesii (Haemodoraceae)

Bronwyn M Ayre et al. Appl Plant Sci. .

Abstract

Premise of the study: Microsatellites were developed for the future assessment of population genetic structure, mating system, and dispersal of the perennial kangaroo paw, Anigozanthos manglesii (Haemodoraceae), and related species.

Methods and results: Using a Personal Genome Machine (PGM) semiconductor sequencer, ca. 4.03 million sequence reads were generated. QDD pipeline software was used to identify 190,000 microsatellite-containing regions and priming sites. From these, 90 were chosen and screened using PCR, and 15 polymorphic markers identified. These sites amplified di-, tri-, and pentanucleotide repeats with one to 20 alleles per locus. Primers were also amplified across congeners A. bicolor, A. flavidus, A. gabrielae, A. humilis, A. preissii, A. pulcherrimus, A. rufus, and A. viridis to assess cross-species transferability.

Conclusions: These markers provide a resource for population genetic studies in A. manglesii and other species within the genus.

Keywords: Anigozanthos; Catspaw; Haemodoraceae; Kangaroo Paw; microsatellite primers.

PubMed Disclaimer

References

    1. Carlson J. E., Tulsieram L. K., Glaubitz J. C., Luk V. M. K., Kauffeldt C., Rutledge R. 1991. Segregation of random amplified DNA markers in F1 progeny of conifers. Theoretical and Applied Genetics 83: 194–200. - PubMed
    1. Hopper S. 1993. Kangaroo Paws and Catspaws. Department of Conservation and Land Management, Perth, Western Australia, Australia.
    1. Krauss S. L., Phillips R. D., Karron J. D., Johnson S. D., Roberts D. G., Hopper S. D. 2017. Novel consequences of bird pollination for plant mating. Trends in Plant Science 22: 395–410. - PubMed
    1. Meglécz E., Pech N., Gilles A., Dubut V., Hingamp P., Trilles A., Grenier R., Martin J. F. 2014. QDD version 3.1: A user-friendly computer program for microsatellite selection and primer design revisited: Experimental validation of variables determining genotyping success rate. Molecular Ecology Resources 14: 1302–1313. - PubMed
    1. Peakall R., Smouse P. E. 2006. GenAlEx 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295. - PMC - PubMed

LinkOut - more resources