Some aspects of the iodine metabolism of the giant kelp Macrocystis pyrifera (phaeophyceae)

Abstract

Despite its paramount role in the functioning of coastal ecosystems, relatively little is known about halogen metabolism in giant kelp (Macrocystis pyrifera). This is an important shortcoming given the potential implications for marine and atmospheric chemical processes in the wide distribution range of Macrocystis. The work presented here constitutes the first in depth investigation of the uptake, efflux, and of the physiological function of iodide in this important kelp species. Iodide uptake and efflux rates were measured in adult sporophytes of Macrocystis under normal and stressed (exogenous hydrogen peroxide and an elicitor-triggered oxidative burst) conditions. Kelp tissue took up iodide according to Michaelis-Menten type kinetics when incubated in seawater enriched with various concentrations of iodide. Upon the addition of exogenous hydrogen peroxide, simulating oxidative stress, a marked efflux of iodide occurred. In situ generation of hydrogen peroxide was elicited in Macrocystis upon the addition of oligomeric degradation products of alginate as well as arachidonic acid and methyl jasmonate constituting a defensive oxidative burst that could be linked to iodine accumulation. H₂O₂ was detected at the single cell level using dichlorohydrofluorescein diacetate, a fluorogenic probe capable of detecting intracellular H₂O₂. When assayed for vanadium haloperoxidase activity, several bromoperoxidase isoforms were detected as well as a single iodoperoxidase. Altogether, the results of this study show that Macrocystis has an elaborate iodine metabolism, which is likely significant for impacting iodine speciation in seawater around kelp beds and for volatile halogen emissions into the coastal atmosphere.

Keywords: Haloperoxidase; Hydrogen peroxide; Iodine; Macrocystis; Marine algae.