Influence of Quercetin in the Temporal Regulation of Redox Homeostasis in Drosophila melanogaster

Abstract

Numerous biological processes are governed by the biological clock. Studies using Drosophila melanogaster (L.) are valuable that could be of importance for their effective applications on rodent studies. In this study, the beneficial role of quercetin (a flavonoid) on H2O2 induced stress in D. melanogaster was investigated. D. melanogaster flies were divided into four groups (group I - control, group II - H2O2 (acute exposure), group III - quercetin, and group IV - quercetin + H2O2 treated). Negative geotaxis assay, oxidative stress indicators (protein carbonyls, thiobarbituric reactive substances [TBARS]), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione-S-transferase [GST], glutathione peroxidase, and reduced glutathione [GSH]) were measured at 4 h intervals over 24 h and temporal expression of heat shock protein-70 (Hsp70), Upd1 (homolog of IL-6 in Drosophila), and nitric oxide synthase (Nos) was analyzed by Western blotting. Groups II and IV showed altered biochemical rhythms (compared with controls). Decreased mesor values of negative geotaxis, SOD, CAT, GST, and GSH were noticed in H2O2, increased mesor of oxidative stress indicators (TBARS and protein carbonyl content) and a reversibility of the rhythmic characteristics were conspicuous after quercetin treatment. The expression levels of Hsp70, Upd1, and Nos were noticeably maximum at 04:00. Significant elevation of expression by H2O2 was nearly normalized by quercetin treatment. The possible mechanism by which quercetin modulates oxidant-antioxidant imbalance under oxidative stress could be ascribed to the modulation of the rhythmic properties. Our results will be helpful to understand the molecular interlink between circadian rhythm and oxidative stress mechanism.

Keywords: Drosophila melanogaster; circadian; oxidative stress; quercetin.

Fig. 1.

Representative cosine fitted curves of 24 h oscillations of negative geotaxis, protein carbonyl, TBARS, SOD, CAT, GST, GPx and GSH in head and body regions in control and experimental groups of flies.

Fig. 1.

Representative cosine fitted curves of 24 h oscillations of negative geotaxis, protein carbonyl, TBARS, SOD, CAT, GST, GPx and GSH in head and body regions in control and experimental groups of flies.

Fig. 1.

Continued.

Fig. 2.

Temporal expression pattern and plot of expression level of Hsp70 (A), Upd1 (B), and Nos (C) in all the groups of D. melanogaster (F = 6.5; df = 18; P < 0.01 – H₂O₂ group compared with control; F = 7.2; df = 18; P < 0.01 – quercetin + H₂O₂ group compared with H₂O₂ group).