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. 2017 Sep 15;216(suppl_4):S555-S559.
doi: 10.1093/infdis/jix001.

Cross-Reactive Antibody Responses to Novel H5Nx Influenza Viruses Following Homologous and Heterologous Prime-Boost Vaccination with a Prepandemic Stockpiled A(H5N1) Vaccine in Humans

Min Z Levine  1 , Crystal Holiday  1 , Feng Liu  1 , Stacie Jefferson  1 , Eric Gillis  1 , Abbie R Bellamy  2 , Terrence Tumpey  1 , Jacqueline M Katz  1
Affiliations

Cross-Reactive Antibody Responses to Novel H5Nx Influenza Viruses Following Homologous and Heterologous Prime-Boost Vaccination with a Prepandemic Stockpiled A(H5N1) Vaccine in Humans

Min Z Levine et al. J Infect Dis. .

Abstract

Recently, novel highly pathogenic avian influenza H5Nx viruses (clade 2.3.4.4) caused outbreaks in US poultry. We evaluated the potential of a stockpiled A(H5N1) A/Anhui/1/2005 (clade 2.3.4) vaccine to elicit cross-reactive antibody responses to these emerging viruses. Sera from subjects who received 2 doses of MF59-adjuvanted A/Anhui/1/2005, or 1 dose of MF59-adjuvanted A/Anhui/1/2005 following priming with a clade 1 vaccine were characterized by microneutralization assays and modified hemagglutination inhibition (HI) assays. Only heterologous prime-boost vaccination induced modest cross-reactive HI antibody responses to H5Nx viruses. Heterologous prime-boost may provide a more effective vaccination strategy to broaden the antibody responses to emerging viruses.

Keywords: antibody responses; highly pathogenic avian influenza viruses; pre-pandemic stockpile.

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Figures

Figure 1.
Figure 1.
Hemagglutination inhibition (HI) and microneutralization (MN) antibody responses to H5N1 and H5N8 viruses 28 days following MF59-adjuvanted A/Anhui1 boost. Scatterplot of HI and MN antibody responses to A/Anhui1 (H5N1), and A/Gyr1088-6 (H5N8) 28 days post–A/Anhui1 boost. Error bars indicate 95% confidence interval. *P < .05. Abbreviations: HI, hemagglutination inhibition; MN, microneutralization; PB, Prime-Boost; VN, A/VN/1203.
See this image and copyright information in PMC

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