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. 2017 Sep 22;15(10):296.
doi: 10.3390/md15100296.

Metabolic Engineering of Escherichia coli for Producing Astaxanthin as the Predominant Carotenoid

Qian Lu  1 , Yi-Fan Bu  2 , Jian-Zhong Liu  3
Affiliations

Metabolic Engineering of Escherichia coli for Producing Astaxanthin as the Predominant Carotenoid

Qian Lu et al. Mar Drugs. .

Abstract

Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. A higher ratio of astaxanthin to the total carotenoids is required for efficient astaxanthin production. β-Carotene ketolase and hydroxylase play important roles in astaxanthin production. We first compared the conversion efficiency to astaxanthin in several β-carotene ketolases from Brevundimonas sp. SD212, Sphingomonas sp. DC18, Paracoccus sp. PC1, P. sp. N81106 and Chlamydomonas reinhardtii with the recombinant Escherichia coli cells that synthesize zeaxanthin due to the presence of the Pantoea ananatis crtEBIYZ. The B. sp. SD212 crtW and P. ananatis crtZ genes are the best combination for astaxanthin production. After balancing the activities of β-carotene ketolase and hydroxylase, an E. coli ASTA-1 that carries neither a plasmid nor an antibiotic marker was constructed to produce astaxanthin as the predominant carotenoid (96.6%) with a specific content of 7.4 ± 0.3 mg/g DCW without an addition of inducer.

Keywords: Escherichia coli; astaxanthin; metabolic engineering; β-carotene hydroxylase; β-carotene ketolase.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Pathway for biosynthesizing astaxanthin. DMAPP: Dimethylallyl diphosphate; GPP: Geranyl pyrophosphate; FPP: Farnesyl pyrophosphate; GGPP: Geranylgeranyl pyrophosphate; ispA: FPP synthase gene; crtE: GGPP synthase gene; crtB: Phytoene synthase gene; crtI: Carotene desaturase gene; crtY: Lycopene β-cyclase gene; crtZ: β-carotene hydroxylase gene; crtW: β-carotene ketolase gene.
Figure 2
Figure 2
HPLC analysis of carotenoid products extracted from E. coli ZEAX (pZS-2crtWBsp) (A), E. coli ZEAX (pZS-2crtWBsp, pBAD-crtZ) (B), E. coli ZEAX (pZS-2crtWBsp, pBAD-crtWBsp) (C), E. coli ZEAX-4 (pZS-2crtWBsp) (D), E. coli ASTA (E), E. coli ASTA-1 (F) and standard astaxanthin (G). 1. astaxanthin; 2. Phoenicoxanthin; 3. Canthaxanthin; 4. Lycopene.
See this image and copyright information in PMC

References

    1. Hussein G., Sankawa U., Goto H., Matsumoto K., Watanabe H. Astaxanthin, a carotenoid with potential in human health and nutrition. J. Nat. Prod. 2006;69:443–449. doi: 10.1021/np050354+. - DOI - PubMed
    1. Lee P.C., Schmidt-Dannert C. Metabolic engineering towards biotechnological production of carotenoids in microorganisms. Appl. Microbiol. Biotechnol. 2002;60:1–11. - PubMed
    1. Ye V.M., Bhatia S.K. Pathway engineering strategies for production of beneficial carotenoids in microbial hosts. Biotechnol. Lett. 2012;34:1405–1414. doi: 10.1007/s10529-012-0921-8. - DOI - PubMed
    1. Lemuth K., Steuer K., Albermann C. Engineering of a plasmid-free Escherichia coli strain for improved in vivo biosynthesis of astaxanthin. Microb. Cell Fact. 2011;10:29. doi: 10.1186/1475-2859-10-29. - DOI - PMC - PubMed
    1. Zhou P.P., Ye L.D., Xie W.P., Lv X.M., Yu H.W. Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt. Appl. Microbiol. Biotechnol. 2015;99:8419–8428. doi: 10.1007/s00253-015-6791-y. - DOI - PubMed

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