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. 2017 Sep 22;7(1):12179.
doi: 10.1038/s41598-017-11777-x.

Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity

Ying Zhao  1 , Guofang Feng  2 , Jie Zhang  1 , Renjie Gong  1 , Changming Cai  1 , Limin Feng  3
Affiliations

Effects of preanalytical frozen storage time and temperature on screening coagulation tests and factors VIII and IX activity

Ying Zhao et al. Sci Rep. .

Abstract

Preanalytical quality control of blood samples is critical for tests of coagulation function and coagulation factor activity. Preanalytical storage time and temperature are the main variables. We investigated the effects of preanalytical frozen storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT)/international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in frozen plasma. Samples (n = 144) were randomly and equally divided into four groups (storage at -80 °C or -20 °C) and analysed by CS5100 or CA7000 coagulation analysers. Baseline values and results after storage for 15 days, 1 month, 3 months, 6 months, and 1 year were measured after thawing. Mean percent changes and scatter plots were used to determine clinically relevant differences. The stabilities of coagulation tests and coagulation factor activities measured by the CS5100 system were consistent with those measured by the CA7000 system. At -80 °C, assessment samples of PT/INR, Fbg, and TT can be safely stored for 1 year, APTT for 6 months, and FVIII:C and FIX:C for 1 month. At -20 °C, samples of Fbg and TT can be stored for 1 year, PT/INR and FIX:C for 1 month, and APTT and FVIII:C for 15 days.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Percent changes of PT in samples stored for 15 days, 1 month, 3 months, 6 months, and 1 year and detected by the CS5100 and CA7000 systems. 1: Samples stored at −80 °C and analysed by the CS5100 system; 2: samples stored at −20 °C and analysed by the CS5100 system; 3: samples stored at −80 °C and analysed by the CA7000 system; 4: samples stored at −20 °C and analysed by the CA7000 system. The two dotted lines in the figures represent plus or minus 10% changes.
Figure 2
Figure 2
Percent changes of APTT in samples stored for 15 days, 1 month, 3 months, 6 months, and 1 year and detected by the CS5100 and CA7000 systems.1: Samples stored at −80 °C and analysed by the CS5100 system; 2: samples stored at −20 °C and analysed by the CS5100 system; 3: samples stored at −80 °C and analysed by the CA7000 system; 4: samples stored at −20 °C and analysed by the CA7000 system. The two dotted lines in the figures represent plus or minus 10% changes.
Figure 3
Figure 3
Percent changes of Fbg in samples stored for 15 days, 1 month, 3 months, 6 months, and 1 year and detected by the CS5100 and CA7000 systems. 1: Samples stored at −80 °C and analysed by the CS5100 system; 2: samples stored at −20 °C and analysed by the CS5100 system; 3: samples stored at −80 °C and analysed by the CA7000 system; 4: samples stored at −20 °C and analysed by the CA7000 system. The two dotted lines in the figures represent plus or minus 10% changes.
Figure 4
Figure 4
Percent changes of TT in samples stored for 15 days, 1 month, 3 months, 6 months, and 1 year and detected by the CS5100 and CA7000 systems. 1: Samples stored at −80 °C and analysed by the CS5100 system; 2: samples stored at −20 °C and analysed by the CS5100 system; 3: samples stored at −80 °C and analysed by the CA7000 system; 4: samples stored at −20 °C and analysed by the CA7000 system. The two dotted lines in the figures represent plus or minus 10% changes.
Figure 5
Figure 5
Percent changes of FVIII:C and FIX:C in samples stored for 15 days, 1 month, 3 months, 6 months, and 1 year and detected by the CS5100 and CA7000 systems. 1: Samples stored at −80 °C and analysed by the CS5100 system; 2: samples stored at −20 °C and analysed by the CS5100 system; 3: samples stored at −80 °C and analysed by the CA7000 system; 4: samples stored at −20 °C and analysed by the CA7000 system. The two dotted lines in the figures represent plus or minus 10% changes.
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