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. 2017 Nov 13;56(46):14556-14560.
doi: 10.1002/anie.201708277. Epub 2017 Oct 9.

Luciferases with Tunable Emission Wavelengths

Julien Hiblot  1   2 , Qiuliyang Yu  1   2 , Marina D B Sabbadini  1 , Luc Reymond  1 , Lin Xue  1   2 , Alberto Schena  1 , Olivier Sallin  1 , Nicholas Hill  1 , Rudolf Griss  1 , Kai Johnsson  1   2
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Luciferases with Tunable Emission Wavelengths

Julien Hiblot et al. Angew Chem Int Ed Engl. .

Abstract

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

Keywords: bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring.

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