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Comparative Study
. 2017 Nov;31(6):1796-1803.
doi: 10.1111/jvim.14834. Epub 2017 Sep 23.

Influence of Various Factors on Circulating 25(OH) Vitamin D Concentrations in Dogs with Cancer and Healthy Dogs

N Weidner  1 , J P Woods  1 , P Conlon  2 , K A Meckling  3 , J L Atkinson  4 , J Bayle  5 , A J Makowski  6 , R L Horst  6 , A Verbrugghe  1
Affiliations
Comparative Study

Influence of Various Factors on Circulating 25(OH) Vitamin D Concentrations in Dogs with Cancer and Healthy Dogs

N Weidner et al. J Vet Intern Med. 2017 Nov.

Abstract

Background: Low blood 25-hydroxyvitamin D (25(OH)D) concentrations have been associated with cancer in dogs. Little research has examined what other factors may affect 25(OH)D concentrations.

Objectives: (1) To determine whether the presence of cancer (lymphoma, osteosarcoma, or mast cell tumor [MCT]) in dogs is associated with plasma 25(OH)D concentrations and (2) identify other factors related to plasma 25(OH)D concentrations in dogs.

Animals: Dogs newly diagnosed with osteosarcoma (n = 21), lymphoma (n = 27), and MCT (n = 21) presented to a tertiary referral oncology center, and healthy, client-owned dogs (n = 23).

Methods: An observational study design was used. Dietary vitamin D intake, sex, age, body condition score (BCS), muscle condition score (MCS), and plasma concentrations of 25(OH)D, 24,25-dihydroxyvitamin D (24,25(OH)2 D) (a marker of CYP24A1 activity), as well as ionized calcium (ICa), parathyroid hormone, and parathyroid hormone-related protein concentrations were measured. An analysis of covariance was used to model plasma 25(OH)D concentrations.

Results: Cancer type (P = 0.004), plasma 24,25(OH)2 D concentrations (P < 0.001), and plasma ICa concentrations (P = 0.047) had significant effects on plasma 25(OH)D concentrations. Effects of age, sex, body weight, BCS, MCS, and plasma PTH concentrations were not identified. A significant interaction between ICa and cancer was found (P = 0.005). Plasma 25(OH)D concentrations increased as ICa concentrations increased in dogs with cancer, whereas plasma 25(OH)D concentrations decreased as ICa concentrations increased in healthy dogs.

Conclusions and clinical importance: Results support a relationship between cancer and altered vitamin D metabolism in dogs, mediated by plasma ICa concentrations. The CYP24A1 activity and plasma ICa should be measured in studies examining plasma 25(OH)D concentrations in dogs.

Keywords: 24,25-dihydroxyvitamin D; 25-hydroxyvitamin D; Lymphoma; Osteosarcoma.

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Conflict of interest statement

Julie Bayle is a paid employee of Royal Canin. Adronie Verbrugghe is the Royal Canin Veterinary Diets Endowed Chair in Canine and Feline Clinical Nutrition at the Ontario Veterinary College.

Figures

Figure 1
Figure 1
Statistical model of relationship between plasma ICa concentration (mmol/L) and plasma 25(OH)D (ng/mL) concentrations at a plasma 24,25(OH)2D concentration of 24 ng/mL in healthy dogs and those with cancer. RMSE = 0.1036. Healthy n = 23, osteosarcoma (OSA) n = 21, lymphoma (LSA) n = 27, mast cell tumor (MCT) n = 21, 25(OH)D = 25‐hydroxyvitamin D, ICa = ionized calcium.
Figure 2
Figure 2
Statistical model of relationship between plasma 24,25(OH)2D concentration (ng/mL) and plasma 25(OH)D (ng/mL) concentrations at a plasma ICa concentration of 1.33 mmol/L in healthy dogs and those with cancer. RMSE = 0.1036. Healthy n = 23, osteosarcoma (OSA) n = 21, lymphoma (LSA) n = 27, mast cell tumor (MCT) n = 21; 25(OH)D = 25‐hydroxyvitamin D, 24,25(OH)2D = 24,25‐dihydroxyvitamin D.
See this image and copyright information in PMC

References

    1. Bronson RT. Variation in age at death of dogs of different sexes and breeds. Am J Vet Res 1982;43:2057–2059. - PubMed
    1. Adams VJ, Evans KM, Sampson J, Wood JL. Methods and mortality results of a health survey of purebred dogs in the UK. J Small Anim Pract 2010;51:512–524. - PubMed
    1. Canadian Cancer Society's Advisory Committee on Cancer Statistics . Canadian Cancer Statistics 2015. Toronto, ON: Canadian Cancer Society; 2015.
    1. World Cancer Research Fund/American Institute for Cancer Research . Food, Nutrition, Physical Activity, and the Prevention of Cancer: A Global Perspective. Washington, DC: World Cancer Research Fund/American Institute for Cancer Research; 2007.
    1. Kushi LH, Doyle C, McCullough M, et al. American Cancer Society Guidelines on nutrition and physical activity for cancer prevention: Reducing the risk of cancer with healthy food choices and physical activity. CA Cancer J Clin 2012;62:30–67. - PubMed

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