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. 1987 Sep;19(9):476-82.
doi: 10.1007/BF01675417.

gamma-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin

G I Murray  1 , M D Burke, S W Ewen
Affiliations

gamma-Glutamyl transpeptidase demonstrated in tissue sections embedded in glycol methacrylate resin

G I Murray et al. Histochem J. 1987 Sep.

Abstract

A method is described for the histochemical demonstration of gamma-glutamyl transpeptidase in tissue sections embedded in glycol methacrylate at low temperature. Enzyme activity was preserved by a short (3 h) fixation of tissue in 4% paraformaldehyde at 4 degrees C prior to embedding at 4 degrees C. Tissue embedded in glycol methacrylate combined good morphology with accurate enzyme localization. Blocks of the embedded tissue could be stored at room temperature for at least 3 months without loss of enzyme activity. The resin is non-fluorescent, allowing the use of the fluorescent coupling agent 5-nitrosalicylaldehyde to visualize the reaction product.

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