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. 2017 Nov;67(11):4397-4404.
doi: 10.1099/ijsem.0.002303. Epub 2017 Sep 25.

Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider

Laura B Goodman  1 , Marie R Lawton  2 , Rebecca J Franklin-Guild  1 , Renee R Anderson  1 , Lynn Schaan  3 , Anil J Thachil  1 , Martin Wiedmann  2 , Claire B Miller  3   4 , Samuel D Alcaine  2 , Jasna Kovac  5   2
Affiliations

Lactococcus petauri sp. nov., isolated from an abscess of a sugar glider

Laura B Goodman et al. Int J Syst Evol Microbiol. 2017 Nov.

Abstract

A strain of lactic acid bacteria, designated 159469T, isolated from a facial abscess in a sugar glider, was characterized genetically and phenotypically. Cells of the strain were Gram-stain-positive, coccoid and catalase-negative. Morphological, physiological and phylogenetic data indicated that the isolate belongs to the genus Lactococcus. Strain 159469T was closely related to Lactococcus garvieae ATCC 43921T, showing 95.86 and 98.08 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively. Furthermore, a pairwise average nucleotide identity blast (ANIb) value of 93.54 % and in silico DNA-DNA hybridization value of 50.7 % were determined for the genome of strain 159469T, when compared with the genome of the type strain of Lactococcus garvieae. Based on the data presented here, the isolate represents a novel species of the genus Lactococcus, for which the name Lactococcus petauri sp. nov. is proposed. The type strain is 159469T (=LMG 30040T=DSM 104842T).

Keywords: Lactococcus; Marsupialia; Streptococcaceae; aquaculture; farms; genome.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Rooted 16S rRNA gene maximum-likelihood tree of Lactococcus petauri sp. nov. 159469T, 16 type strains of species and subspecies of the genus Lactococcus, and Escherichia coli K-12 as an outgroup. The tree was reconstructed in RAxML v. 8, using the GTRGAMMAI substitution model and 1000 bootstrap repetitions. Numbers at nodes indicate percentage bootstrap support. Bar, 0.2 substitutions per site. L. petauri sp. nov. 159469T (NCBI accession number KY548925) is presented in bold type.
Fig. 2.
Fig. 2.
Core genome single-nucleotide polymorphism (SNP) based maximum-likelihood tree including Lactococcus petauri sp. nov. 159469T and seven type strains of species and subspecies of the genus Lactococcus. The tree was reconstructed with the general time-reversible substitution model and 1000 bootstrap repetitions in RAxML using core genome SNPs identified by kSNP. The tree was rooted by midpoint. Numbers at nodes indicate percentage bootstrap support. Bar, 0.3 substitutions per site. L. petauri sp. nov. 159469T (NCBI accession numbers SRR5220185 and MUIZ00000000) is presented in bold type.
Fig. 3.
Fig. 3.
Pairwise average nucleotide identity blast (ANIb) values for Lactococcus petauri sp. nov. 159469T, and seven type strain of species and subspecies of the genus Lactococcus. ANIb values are presented as 100 % – ANIb. The horizontal line indicates the 95 % ANIb species cut-off [19].
Fig. 4.
Fig. 4.
Rooted rpoB neighbour-joining tree of Lactococcus petauri sp. nov. 159469T, 15 type strains of species and subspecies of the genus Lactococcus. The tree was reconstructed in mega version 6.0, using the Tamura 3-parameter model and 1000 bootstrap repetitions. Bar, 0.05 substitutions per site. Lactococcus petauri sp. nov. 159469T (NCBI accession number MF141900) is presented in bold type.
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