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. 2017 Sep 27;10(10):1139.
doi: 10.3390/ma10101139.

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

Yaping He  1 , Jianbin Zheng  2 , Bini Wang  3 , Hongjiang Ren  4
Affiliations

Double Biocatalysis Signal Amplification Glucose Biosensor Based on Porous Graphene

Yaping He et al. Materials (Basel). .

Abstract

Controllable preparation of nanopores to promote the performance of electrochemical biosensing interfaces has become one of the researching frontiers in biosensing. A double biocatalysis signal amplification of glucose biosensor for the study of electrochemical behaviors of glucose oxidase (GOx) was proposed by using horseradish peroxidase biosynthesized porous graphene (PGR) as the platform for the biocatalytic deposition of gold nanoparticles (AuNPs). The biosensor showed a linear range from 0.25 to 27.5 μM with a detection limit of 0.05 μM (S/N = 3) towards glucose. Furthermore, the proposed AuNPs/GOx-PGR modified glassy carbon electrode (AuNPs/GOx-PGR/GCE) achieved direct electron transfer of GOx.

Keywords: Au nanoparticles; biological amplification; glucose oxidase; horseradish peroxidase; porous graphene.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
SEM images of product with different reaction concentrations of graphene oxide (GO) for 15 days: (A) 1 mg/mL; (B) 2 mg/mL; (C) 4 mg/mL and (D) 6 mg/mL.
Figure 2
Figure 2
TEM images of product with reaction concentrations of GO 2 mg/mL for 15 days with low (A) and high order (B).
Figure 3
Figure 3
Plots of peak current vs. accumulation time.
Figure 4
Figure 4
CVs of glucose oxidase (GOx)-induced formation of gold nanoparticles (AuNPs) for increasing concentrations of glucose. Glucose concentration ranges: (a) 0.25 μM; (b) 10 μM; (c) 20 μM; (d) 25 μM and (e) 27.5 μM. Inset: plot of peak current vs. glucose concentration. Reaction solution: a stirred 0.1 M phosphate buffered saline (PBS) solution containing 0.2 mM HAuCl4 and different concentrations of glucose.
Figure 5
Figure 5
CVs of the AuNPs/GOx–PGR/GCE (glassy carbon electrode) in N2 saturated PBS with different scan rates (from a to n: 100, 150, 200, 250, 300, 350, 400, 450, 500, 600, 700, 800, 900, 1000 mV/s).
See this image and copyright information in PMC

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