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. 2017 Dec;55(12):3405-3410.
doi: 10.1128/JCM.00893-17. Epub 2017 Sep 27.

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

Kohei Uechi  1   2 , Tatsuya Tada  3   4 , Kayo Shimada  4 , Kyoko Kuwahara-Arai  3 , Momoko Arakaki  1 , Takaaki Tome  1 , Isamu Nakasone  5 , Shiro Maeda  1 , Teruo Kirikae  6   4 , Jiro Fujita  2
Affiliations

A Modified Carbapenem Inactivation Method, CIMTris, for Carbapenemase Production in Acinetobacter and Pseudomonas Species

Kohei Uechi et al. J Clin Microbiol. 2017 Dec.

Abstract

The carbapenem inactivation method (CIM) and modified CIM (mCIM) are simple and economical phenotypic screening methods for detecting carbapenemase production in Gram-negative bacteria. Although the mCIM has been recommended by the Clinical and Laboratory Standards Institute, both the CIM and mCIM have limitations. This study describes another modified CIM, called CIMTris, in which carbapenemase was extracted from bacteria with 0.5 M Tris-HCl (pH 7.6) buffer. The ability of the CIMTris to detect carbapenemase production was examined in Acinetobacter and Pseudomonas species. The CIMTris had an overall sensitivity of 97.6% and an overall specificity of 92.6%, whereas the mCIM had a sensitivity of 45.1% and a specificity of 100% for the isolates tested. These findings indicate that the CIMTris is useful for detecting carbapenemase production in Acinetobacter and Pseudomonas species.

Keywords: Acinetobacter species; Pseudomonas species; carbapenem inactivation method; carbapenem resistance.

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