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. 2017 Sep 12:8:1637.
doi: 10.3389/fmicb.2017.01637. eCollection 2017.

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

Sandrine Baron  1   2 , Emeline Larvor  1   2 , Séverine Chevalier  2   3 , Eric Jouy  1   2 , Isabelle Kempf  1   2 , Sophie A Granier  4 , Jean Lesne  2   3
Affiliations

Antimicrobial Susceptibility among Urban Wastewater and Wild Shellfish Isolates of Non-O1/Non-O139 Vibrio cholerae from La Rance Estuary (Brittany, France)

Sandrine Baron et al. Front Microbiol. .

Abstract

The early 2000s marked the end of the Golden age of the antibiotics and the beginning of the awareness on the potential threat to human health due to the dissemination of antimicrobial resistance. As a base-line study, we investigated the antimicrobial susceptibility of 99 strains of non-O1/non-O139 Vibrio cholerae isolated from wastewater and shellfish in 2000/2001 within La Rance estuary (Brittany, France). All isolates were susceptible to amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, nalidixic acid, ciprofloxacin, norfloxacin, amikacin, gentamicin, tetracycline, doxycycline, trimethoprim-sulfamethoxazole, and erythromycin. The only resistances were to streptomycin, sulfonamides and ampicillin: 54.6% of the isolates had acquired resistance to at least one antimicrobial agent among them and only six isolates from cockles were multidrug resistant. On the basis of the distribution of a limited selection of resistance associated genes, our study shows that V. cholerae can constitute an environmental reservoir for these genes. However, none of our isolates harbored integron. This result casts doubt on the capacity of non-O1/non-O139 V. cholerae to acquire resistance-associated genes in such context, and on its potential role of indicator of the dissemination of antimicrobial resistance in the aquatic environment.

Keywords: Vibrio cholerae non-O1/non-O139; antimicrobial resistance; estuary; wastewater; wild shellfish.

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Figures

Figure 1
Figure 1
Map of Rance estuary.
Figure 2
Figure 2
Densities of V. cholerae, E. coli and intestinal Enterococci in treated wastewater. Densities of E. coli and intestinal Enterococci were expressed by 100 mL of water and V. cholerae per 1 liter of water.
Figure 3
Figure 3
Distribution of resistance profiles among the 99 isolates of non-O1/non-O139 Vibrio cholerae.
Figure 4
Figure 4
Distribution of diffusion zone diameters: (A) doxycycline (30 μg); (B) erythromycin (15 μg).
See this image and copyright information in PMC

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