Phenolic extracts and amino acids content from Cucumeropsis mannii naudin and Citrullus lanatus inhibit relevant enzymes of erectile dysfunction in rat's penile tissue

Abstract

•A focused on the erectogenic potentials of two tropical pumpkin seeds was established.•The antioxidant properties of these seed varieties were also discussed.•The inhibitory effects of the seeds on key enzymes relevant to ED were critically explored.•The potentials of the seeds to manage ED were recommended.

Fig. 1

Interaction of extracts from some pumpkin seeds with arginase activity on corpus cavernosum.

Fig. 2

Interaction of extracts from some pumpkin seeds with phosphodiesterase 5′ activity on corpus cavernosum.

Fig. 3

DPPH free radical scavenging ability of extracts from some pumpkin seeds.

Fig. 4

OH* radical scavenging abilities of extracts from some pumpkin seeds.

Fig. 5

Inhibition of Fe²⁺ induced lipid peroxidation in rat's corpus cavernosum by extracts of some pumpkin seeds.

Fig. 6

Inhibition of SNP induced lipid peroxidation in rat's corpus cavernosum by extracts of some pumpkin seeds.

Fig. 7

Cu²⁺ Chelating ability of extracts from some pumpkin seeds in-vitro.

Fig. 8

Fe²⁺ Chelating ability of extracts from some pumpkin seeds in-vitro.

Fig. 9

Representative HPLC–DAD chromatography profile of phenolic constituents of (B) Cucumeropsis mannii naudin and (C) Citrullus lanatus. Peak assignment: (1) gallic acid, (2) caffeic acid, (3) p-coumaric acid, (4) rutin, (5) luteolin, (6) quercetin, (7) quercitrin, (8) kaempferol and (9) catechin.