Development of an analytical method for identification of Aspergillus flavus based on chemical markers using HPLC-MS

Abstract

Aspergillus flavus is a filamentous fungus found in nature and characterized by the production of bright and colourful colonies. It grows on different substrates, producing secondary metabolites and, if present in foodstuffs, can be a source of health problems for humans and animals, as well as causing economic losses. Traditional methods for fungal identification are based on morphological characteristics, requiring specialists and being very time-consuming. The development of analytical alternatives might have advantages such as greater efficiency, more reproducibility and be less time-consuming. Thus, a qualitative analytical method to detect Aspergillus flavus in food samples, based on the identification of fungal chemical markers by HPLC-MS, was developed. The method comprises methanol extraction followed by HPLC-MS analysis, and was able to identify 14 fungus secondary metabolites, namely aflatoxin B1, aflatoxin G1, aspergillic acid, aspyrone, betaine, chrysogine, deacetyl parasiticolide A, flufuran, gregatin B, hydroxysydonic acid, nicotinic acid, phomaligin A, spinulosin and terrein.

Keywords: Aflatoxin B1 (PubChem CID: 186907); Aflatoxin G1 (PubChem CID: 14421); Aflatoxins; Aspergillic acid (PubChem CID: 10272); Aspyrone (PubChem CID: 167653); Chrysogine (PubChem CID: 63145); Fungi; Gregatin B (PubChem CID: 53304446); Mycotoxins; Phomaligin A (PubChem CID: 101922009); Screening test; Secondary metabolites; Spinulosin (PubChem CID: 66556); Terrein (PubChem CID: 6436830).