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. 2014 Nov 4:1:915-922.
doi: 10.1016/j.toxrep.2014.10.022. eCollection 2014.

Antioxidant effect of pomegranate against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats

Anahita Aboonabi  1 , Asmah Rahmat  1 , Fauziah Othman  2
Affiliations

Antioxidant effect of pomegranate against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats

Anahita Aboonabi et al. Toxicol Rep. .

Abstract

Oxidative stress attributes a crucial role in chronic complication of diabetes. The aim of this study was to determine the most effective part of pomegranate on oxidative stress markers and antioxidant enzyme activities against streptozotocin-nicotinamide (STZ-NA)-induced diabetic rats. Male Sprague-Dawley rats were randomly divided into six groups. Experimental diabetes was induced by a single intraperitoneal injection (i.p), 15 min after the i.p administration of NA. Diabetic rats showed significant increase in plasma glucose level, and the significant decrease in plasma insulin level. The activities of antioxidant enzymes such as total antioxidant status (TAS), superoxide dismutase (SOD), and catalase (CAT) reduced while the levels of biomarkers of oxidative stress such as gamma-glutamyle transferase (GGT), and malondialdehyde (MDA) increased in diabetic control rats as compared to normal control rats. Oral treatment with pomegranate seed-juice for 21 days demonstrated significant protective effects on all the biochemical parameters studied. Besides, biochemical findings were supported by histopathological study. These results revealed that pomegranate has potential protective effect against oxidative stress induced diabetic rats.

Keywords: Antioxidant; Diabetes; Dimethyl sulfoxide (PubChem CID: 679); Eosin (PubChem CID: 11048); Glibenclamide (PubChem CID: 3488); Hematoxyline (PubChem CID: 442514); Isonicotinamide (PubChem CID: 15047); Oxidative Stress; Pomegranate; Sodium citrate (PubChem CID: 6224); Streptozotocin; Streptozotocin (PubChem CID: 29327).

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Figures

Fig. 1
Fig. 1
Combined changes in the levels of plasma glucose and insulin in normal diabetic rats, Each values is mean ± S.E.M. for 8 rats in each group. a–dIn each bar means different significantly at p < 0.05. NC: normal control, DC: diabetic control, PS: pomegranate seed, PJ: pomegranate juice, PSJ: pomegranate seed-juice, GC: Glibenclamide.
Fig. 2
Fig. 2
Effect of pomegranate on plasma MDA (A) and GGT (B) plasma in controls and treatment rats, each value is mean ± S.E.M. for eight rats in each group. a–eIn each bar means different significantly at p < 0.05. NC: normal control, DC: diabetic control, PS: pomegranate seed, PJ: pomegranate juice, PSJ: pomegranate seed-juice, GC: Glibenclamide.
Fig. 3
Fig. 3
Liver micrograph sections of normal and diabetic rats treated with PSJ. (A) Normal control shows normal architecture of the hepatocytes around the central vein with sinusoidal cards around the central vein and portal tracts (H&E X20). (B) Diabetic section untreated shows dilated sinusoids (black arrow) and dilated central vein (H&E X20). (C) Micrograph with higher magnification shows inflammation (red arrows) around central vein and necrosis in the hepatocytes (H&E X40). (D) Diabetic rats treated with PSJ shows near normal hepatocytes, mild sinusoidal dilatation without any inflammation around central vein when compared to diabetic liver (H&E X20).
See this image and copyright information in PMC

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