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. 2017 Dec 1;21(4):313-320.
doi: 10.5935/1518-0557.20170056.

Alterations in expression of endometrial milk fat globule-EGF factor 8 (MFG-E8) and leukemia inhibitory factor (LIF) in patients with infertility and endometriosis

Carla Regina Schmitz  1 , Sergio Oehninger  2 , Vanessa Krebs Genro  3 , Neelima Chandra  2 , Frank Lattanzio  4 , Liang Yu  2 , João Sabino Cunha-Filho  1   3
Affiliations

Alterations in expression of endometrial milk fat globule-EGF factor 8 (MFG-E8) and leukemia inhibitory factor (LIF) in patients with infertility and endometriosis

Carla Regina Schmitz et al. JBRA Assist Reprod. .

Abstract

Objective: The aim of this study was to compare the endometrial expression of milk fat globule-EGF factor 8 (MFG-E8), its receptor integrin αvβ3, and leukemia inhibitory factor (LIF) in patients with endometriosis and infertility and in healthy fertile patients during the window of implantation.

Methods: Five patients with peritoneal endometriosis and infertility (case group) and four healthy fertile patients (control group) were recruited. All patients were either diagnosed with or ruled out for endometriosis by laparoscopic surgery; the case group underwent surgery for infertility investigation and the control group for tubal ligation. Endometrial biopsies were performed in all patients during the window of implantation (LH+8 to LH+10), and then the samples were analyzed by immunochemistry for MFG-E8, integrin αvβ3, and LIF.

Results: In patients with endometriosis and infertility, expression of MFG-E8 was significantly increased in the glandular epithelium when compared to healthy fertile patients (p<0.001). Moreover, LIF expression was lower in patients with endometriosis and infertility (p<0.05). Nevertheless, we found no difference in integrin αvβ3 expression between the groups (p=0.084).

Conclusion: This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. Moreover, LIF is also diminished in the endometrium of these patients as shown before.

Keywords: LIF; MFG-E8; endometriosis; human endometrium; integrin αvβ3.

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Figures

Figure 1
Figure 1
Immunohistochemical localization of MFG-E8, integrin αvβ3, and LIF in the human endometrium. Representative images of: (A) negative control from the control group (endometriosis and infertility), (B) and from the case group (healthy fertile patients); (C) MFG-E8 staining from the control group, (D) and from the case group; (E) Integrin αvβ3 staining from the control group, (F) and from the case group; and (G) LIF staining from the control group and from the case group (H).
Figure 2
Figure 2
MFG-E8 (A), integrin αvβ3 (B) and LIF (C) HSCORE in healthy fertile patients (control group) and in patients with endometriosis and infertility. **p<0.001 compared with controls. *p<0.05 compared with controls.
See this image and copyright information in PMC

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